Background: In plant breeding program to produce hybrid varieties, pair of male sterile and restorer fertility lines are required. Differentiation of lines possessing restorer fertility allele from the lines lacking it remove the need for the progeny test, and thus reducing the time and the cost in the hybrid production procedure. Canola breeding program in Iran has concentrate...

Rice restorer lines play an important role in three-line hybrid rice production. Previous research based on molecular tagging has suggested that the restorer lines used widely today have narrow genetic backgrounds. However, patterns of genetic variation at a genome-wide scale in these restorer lines remain largely unknown. The present study performed re-sequencing and genome-wide variation anal...

Identification of maintainer and restorers from rice germplasm through test crossing is the initial step in three line heterosis breeding. Maintainer lines can be converted into cytoplasmic male sterile lines to utilize in development of rice hybrids. Restorer lines can be used as males to produce F1 hybrids. Three hundred F2 plants of the cross KCMS 26A X IET 19886 were grown under aerobic sit...

The combination of cytoplasmic male sterility (CMS) in one parent and a restorer gene (Rf) to restore fertility in another are indispensable for the development of hybrid varieties. To genotype rice lines at the restoring fertility (Rf) loci, 38 lines were crossed with a sterile tester (rfrf) line. Pollen fertility test was performed to identify sterile and fertile F1 hybrids. Seven lines were ...

An experiment was conducted at Bangladesh Rice Research Institute (BRRI), Gazipur during T.Aman season of 2007 with a view to evaluating seven maintainer and 30 restorer lines for yield and yield contributing characters for selecting promising maintainer and restorer lines for developing rice hybrids in our local environment. Significant variations were noticed among the parental lines for all ...

Hybrid rice has contributed significantly to the world food security. Breeding of elite high-yield, strong-resistant broad-spectrum restorer line is an important strategy for hybrid rice in commercial breeding programs. Here, we developed three elite brown planthopper (BPH)-resistant wide-spectrum restorer lines by pyramiding big-panicle gene Gn8.1, BPH-resistant genes Bph6 and Bph9, fertility ...

the present study was carried out with the objective of validating linked ssr markers to rf genes and adopting marker assisted selection (mas) for restorer/non-restorer line detection in wild abortive (wa) type of cytoplasmic male sterility (cms). twelve ssr markers reported to be linked to rf genes were analyzed in the mapping population of nedaa/pajouhesh. among these, three markers, namely, ...

Mitochondrial atpA transcripts were examined in cytoplasmic male sterile (CMS) and fertility restorer lines of CMS (Moricandia arvensis) Brassica juncea. Male sterile flowers had longer atpA transcripts than male fertiles. The mitochondrial atpA region of the CMS line was cloned and sequenced. The 5' and 3' ends of the atpA transcripts of the CMS and the fertility restorer lines were mapped and...

for different agronomic traits in sunflower (helianthus annuus l.) inbred lines were estimated using “line × tester” analysis method. six new restorer lines were crossed with four cms lines as tester in 2006 growing season. the 24 f1 single cross combinations were planted in complete randomized block design with four replications in khoy field research station in 2007 growing season. there were...