The aim of this research was to study the suitability of a manometric respirometric test (OECD 301F) for the determination of biological oxygen demand (BOD) in domestic wastewater. This was done by comparing four different BOD tests (a manometric respirometric test, oxygen sensor determination, iodometric titration, and UV absorption measurement) and by evaluating the BOD(7) value after a short...

The biological oxygen demand (BOD) test is widely used in many wastewater treatment plants. The conventional BOD tests are usually time-consuming and the results are often out of date for process control purposes. The aim of this research was to compare the manometric respirometric test with common chemical methods in the determination of BOD of wastewater from a pulp and paper mills as well as...

in this study, pp films were modified with an organic pro-degradant in different concentrations (1, 2 and 3wt.%), exposed in the first step of degradation to natural ageing for 100 days followed by biodegradation insimulated soil in the respirometric test for 100 days. at the end of the combined degradation process the pp sampleswere characterized according to their morphological and physical p...

Combined respirometric – titrimetric data are presented, resulting from aerobic batch degradation experiments in activated sludge with acetate and dextrose as a substrate. A model including substrate uptake, CO2 production and NH3 uptake for biomass growth is proposed to describe the titrimetric data. Combining respirometric and titrimetric data is more informative compared to the separate data...

The 5-day biochemical oxygen demand (BOD5) test has been used for over a century to evaluate the concentration of biodegradable organic matter in wastewater effluents. The BOD test is a simple batch test, but it is also time consuming, labor intensive, imprecise, and, due to the dilution of the wastewater, takes a relatively long time to complete. Advances in laboratory instruments and lab ware...

The doubly labelled water (DLW) method is an isotope-based technique that is used to measure the metabolic rates of free-living animals. We validated the DLW method for measuring metabolic rates in five rhinoceros auklets (Cerorhinca monocerata) compared with simultaneous measurements using the respirometric method. We calculated the CO(2) production rate of four auklets (mean initial body mass...

Respirometric profiling of isolated mitochondria is commonly used to investigate electron transport chain function. We describe a method for obtaining samples of human Vastus lateralis, isolating mitochondria from minimal amounts of skeletal muscle tissue, and plate based respirometric profiling using an extracellular flux (XF) analyzer. Comparison of respirometric profiles obtained using 1.0, ...

When modelling a biochemical system, the fact that model parameters cannot be estimated exactly stimulates the definition of tests for checking unreliable estimates and design better experiments. The method applied in this paper is a further development from Marsili-Libelli et al. [2003. Confidence regions of estimated parameters for ecological systems. Ecol. Model. 165, 127-146.] and is based ...

Composting is one of the best ways to properly manage the high production of sewage sludge with the aim of obtaining sanitized products with a stable organic matter. The evolution of the stability of a compost consisting of sewage sludge, straw and sawdust, developed under full-scale conditions, was analyzed. The stability was assessed by a respirometric assay performed for 30 days, where the O...

Investigation of mitochondrial function represents an important parameter of cardiac physiology as mitochondria are involved in energy metabolism, oxidative stress, apoptosis, aging, mitochondrial encephalomyopathies and drug toxicity. Given this, technologies to measure cardiac mitochondrial function are in demand. One technique that employs an integrative approach to measure mitochondrial fun...