conclusions after seven consecutive passages of both cell cultures, the h9n2 virus showed similar antigenicity, also no change on viral titer level and virus replication behavior in adaptation was found. the results highlighted the use of a549 cells for efficient virus isolation. results the influenza virus infectivity was not significant difference in these cells in the presence of trypsin. th...

background and aims: infectious laryngotracheitis virus (iltv) infection causes a highly contagious respiratory disease in poultry leads to significant economic losses in the poultry industry. the genomic and antigenic characteristics between virulent and vaccine strains of iltv are very similar. the ability of embryonated chicken neural stem (cns) cell for differential diagnosis of iltv strain...

The mechanism of protein-only prion replication is controversial. A detailed mathematical model of prion replication by nucleated polymerisation is developed, and its parameters are estimated from published data. PrP-res decay is around two orders of magnitude slower than PrP-sen decay, a plausible ratio of two parameters estimated from very different experiments. By varying the polymer breakag...

conclusions taken together, these data reveal that cellular protease of chicken liver cells allows the replication of high yields of h9n2 virus in the absence of trypsin and also cell death in the infected cells is due to necrosis. results the degree of viability was significantly reduced in infected hepatoma cells. observations of caspase activation and cell dna laddering in infected cells wer...

We discuss how the results of presteady-state and steady-state kinetic analysis of the polymerizing and excision activities of herpes simplex virus type 1 (HSV-1) DNA polymerase have led to a better understanding of the mechanisms controlling fidelity of this important model replication polymerase. Despite a poorer misincorporation frequency compared to other replicative polymerases with intrin...