Boosting is a set of methods for the construction of classifier ensembles. The differential feature of these methods is that they allow to obtain a strong classifier from the combination of weak classifiers. Therefore, it is possible to use boosting methods with very simple base classifiers. One of the most simple classifiers are decision stumps, decision trees with only one decision node. This...

Classes are not the best focus for object-oriented design. The most useful components in a design are frameworks – schemes of interaction between objects. These can be worked on by separate authors, kept in libraries, and recombined to make many different end-products. This paper explores the more formal aspects of this approach, within the context of popular OOA/D notations and programming lan...

The feasibility of multi-step, one-flask total synthesis of natural products is demonstrated by cloning and overexpression of the corresponding biosynthetic enzymes from plant and microbial sources. As many as eight or nine enzymes can be recombined in vitro to reach an advanced intermediate in the vitamin B12 pathway.

Xyloglucan endotransglycosylase/hydrolase (XTH) enzymes have played a role in the remodeling of cell wall hemicelluloses. To investigate the function of XTHs in persimmon (Diospyros kaki L.) fruit development and postharvest softening, five cDNAs (DkXTH1 to DkXTH5), whose putative proteins contained the conserved DEIDFEFLG motif of XTH, were cloned. Real time quantitative PCR analysis revealed ...

I have used a plasmid containing two copies of the Saccharmyces cerevisiae his3 gene to study intramolecular homologous recombination in vaccina virus-infected cells. Recombination of the plasmid was monitored by restriction enzyme digestion and Southern blot hybridization in cells infected with representatives from each of 32 complementation groups of temperature-sensitive mutants ts42 and ts1...

Event-related brain potentials (ERPs) were recorded from 24 young adults during a recognition test including Old, New, and Recombined pairs composed of two words studied in different pairs. Recombined pairs called for a response of 'new'. Task difficulty was increased by repetition of some words during the study phase; a subject might study tower/pie, puppet/pie, drill/wreath and bee/wreath (pa...