نتایج جستجو برای: recombinant protein sag1

تعداد نتایج: 1297381  

Journal: :archives of clinical infectious diseases 0
seyedeh mahya marashiyan department of basic sciences, sari agricultural sciences and natural resources university, sari, iran fatemeh moradian department of basic sciences, sari agricultural sciences and natural resources university, sari, iran geita saadatnia department of biotechnology, iranian research organization for science and technology (irost), tehran, iran; department of biotechnology, iranian research organization for science and technology (irost), tehran, iran. tel/fax: +98-2156276636 majid golkar molecular parasitology laboratory, department of parasitology, pasteur institute of iran, tehran, iran

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

Journal: :iranian journal of parasitology 0
n. jalallou dept. of medical laboratory science, school of medicine, army medical science university, tehran, iran. m. bandehpour dept. of biotechnology, shahid beheshti university of medical sciences, tehran, iran. h. khazan dept. of medical parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran. a. haghighi dept. of medical parasitology and mycology, school of medicine, shahid beheshti university of medical sciences, tehran, iran. b. kazemi dept. of biotechnology, shahid beheshti university of medical sciences, tehran, iran and cellular and molecular biology research centers, shahid beheshti university, of medical sciences, tehran, iran.

b ackground: toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. since the aim of this study was to assess the diagnostic utility of recombinant sag1 (rec- sag1) for the detection of toxoplasma -specific igm antibodies in human sera, by an enzyme-linked immunosor...

Journal: :jundishapur journal of microbiology 0
rahman abdizadeh cell and molecular research center, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of medical parasitology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran sharif maraghi department of medical parasitology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; institute of health research, thalassemia and hemoglobinopathy research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran ata a. ghadiri department of immunology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of immunology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-6133330144, fax: +98-6133330145 mehdi tavalla department of medical parasitology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran saeedeh shojaee department of medical parasitology and mycology, school of public health, tehran university of medical sciences tehran, ir iran

background toxoplasmosis is an opportunistic protozoan infection with a high prevalence in a broad range of hosts infecting up to one-third of the world human population. toxoplasmosis leads to serious medical problems in immunocompromised individuals and fetuses and also induces abortion and mortality in domestic animals. therefore, there is a huge demand for the development of an effective va...

2010
N Jalallou M Bandepour H Khazan A Haghighi Sh Abdollahi B Kazemi

BACKGROUND Although some serological tests for the detection of Toxoplasma gondii-specific immunoglobulin are commercially available, better diagnostic tools are needed. The aim of present study was to evaluate the usefulness of the recombinant Toxoplasma gondii SAG1 antigen for the recognition of toxoplasmosis by ELISA. METHODS This study was conducted in Cellular and Molecular Biology Resea...

2012
N Jalallou M Bandehpour H Khazan A Haghighi B Kazemi

BACKGROUND Toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec-SAG1) for the detection of Toxoplasma-specific IgM antibodies in human sera, by an enzyme-linked immunosorbent...

2015
Rahman Abdizadeh Sharif Maraghi Ata A. Ghadiri Mehdi Tavalla Saeedeh Shojaee

BACKGROUND Toxoplasmosis is an opportunistic protozoan infection with a high prevalence in a broad range of hosts infecting up to one-third of the world human population. Toxoplasmosis leads to serious medical problems in immunocompromised individuals and fetuses and also induces abortion and mortality in domestic animals. Therefore, there is a huge demand for the development of an effective va...

2010
Melina Laguía-Becher Valentina Martín Mauricio Kraemer Mariana Corigliano María L Yacono Alejandra Goldman Marina Clemente

BACKGROUND Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG178-322 antigen of Toxoplasma gondii in tobacco leaves. The expression of the tobacco-optimized and native versions of the SAG1 gene was explored by transient expression from the Agrobacterium tumefaciens binary expression vector, which allows targeting the recombinant p...

حسینیان خسروشاهی, کامی, دلیمی‌اصل, عبدالحسین, شریفی, زهره, صلح‌جو , کاوس, غفاری‌فر, فاطمه,

    Background & Aim: Toxoplasmosis is caused by a protozoan parasite called toxoplasma gondii. SAG1 is an antigen which only exists in tachyzoite stage. This antigen which has two glycoforms is a conformational antigen. The aim of this research is to study cloning of SAG1 gene in PTZ57R and transformation of recombinant plasmid in E.coli(TG1 & DH5a). Material & Method: Tachyzoites of T.gondii ...

A. Dalimi, F. Ghafari Far K. Solhjoo Z. Sharifi

  Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...

Journal: :iranian journal of parasitology 0
m (mina) selseleh dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran h keshavarz dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran (crepi), tehran university of medical sciences, tehran, iran m mohebali dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran s shojaee dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran mh modarressi genetic faculty, school of public health, tehran university of medical sciences, tehran, iran mr eshragian epidemiology and biostatistics department, school of public health, tehran university of medical sciences, tehran, iran

background: the assays currently available for the detection of specific anti- toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. the aim of this study was evaluation the recombinant toxoplasma gondii sag1 antigen for the serodiagnosis of acute and chronic toxoplasmosis. methods : this study describes an elisa u...

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