In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...

Background and Aims: Q59L mutant of L-asparaginase enzyme from Escherichia coli (E. coli) has been introduced with lower side effects. This version of the enzyme might have potential applications in the treatment of leukemia patients. We utilized SHuffle T7 strain of E. coli, to produce the mutant enzyme in the presence of chaperone molecules. Materials and Methods: Q59LAsp gene was cloned in...

cell lines derived from mammalian are dominant systems for the production of recombinant therapeutic proteins because of their capacity for correct protein folding, assembly and post-translational modification. in the search of an efficient method for the production of  a recombinant protein using animal cell culture, we investigated the effects of different treatment including fetal calf serum...

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...

Background: Bovine follicle-stimulating hormone (bFSH) is a heterodimer hormone that consists of a common -subunit which noncovalently associated with the hormone-specific -subunit. During the past 15 years, the methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production because it is able to use methanol as a sole carbon and energy source. Th...

background: various prokaryotic and eukaryotic expression systems have been developed for the production of recombinant proteins. in the present study, we used a new protein expression system based on the iranian lizard leishmania, a trypanosomatid protozoan as a host, for the expression of lpg3 gene from leishmania infantum (l.infantum). methods: the lpg3 gene was cloned in the expression cass...

background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...

infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. the expression of heterologous proteins in p.pastoris is fast, simple and inexpensive. in this study, vp2 encoding gene of classical d78 ibdv was amplified using reverse transcription (rt) polymerase chain reaction (pcr) and cloned into ppiczαa vector...