نتایج جستجو برای: recombinant plasmids

تعداد نتایج: 124414  

Journal: :modares journal of medical sciences: pathobiology 2005
fariba ataei hosein ghanbarian ali reza zomorodipour bagher yakhchali

purpose: in order to express human granulocyte-macrophage colony stimulating factor (hgm-csf) under heat shock. materials and methods: two expression plasmids were constructed based on pbc(sk) plasmid. the expression cassettes in the two plasmids are equipped with a 75 base pair fragment, derived from the pl promoter of the bacteriophage lambda (λ). the plasmids also contain a temperature muta...

Journal: :medical journal of islamic republic of iran 0
ali a farhoudi-moghaddam from the molecular biology unit . pasteur institute. tehran. i.r. iran nickolay n domansky a.a ziaee

the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...

Journal: :Cell & gene therapy insights 2022

Transient transfection of plasmids into suspension HEK293 cells is still the most commonly used method for production recombinant adeno-associated virus (rAAV) gene therapies – in this episode, Diane Golebiowski and Marissa Stanvick discuss ways to optimize that process.

Journal: :Technical Tips Online 1997

Journal: :international clinical neurosciences journal 0
reza taherian students' research office, school of medicine, shahid beheshti university of medical sciences, tehran, iran mahdi taherian department of biology, semnan university of medical sciences, semnan, iran

background and purpose: brain-derived neurotrophic factor (bdnf) is present in the hippocampus and cerebral cortex in high concentrations and has an important role in development, maintenance and regeneration of the nervous system. the aim of this study was to produce active bdnf in e.coli using transforming plasmid. methods: to construct an expression plasmid of bdnf, a gene coding for it was ...

A.G. Zhang C.L. Qin M. Cheng M.F. Niu Q. Gong, X. F. Sun

Avian Pasteurella multocida is an agent of fowl cholera. The protective effect achieved through orthodoxvaccines is not ideal. The research on novel vaccines against avian Pasteurella multocida is imperative. Inthis study, the genes encoding outer membrane protein H and A (OmpH and OmpA) were cloned into theeukaryotic expression vector pcDNA3.1(+) and the recombinant plasmids, namely DNA vaccin...

A. Zomorodipour

In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...

Journal: :iranian journal of veterinary research 2013
q. gong c.l. qin m.f. niu m. cheng x. f. sun

avian pasteurella multocida is an agent of fowl cholera. the protective effect achieved through orthodoxvaccines is not ideal. the research on novel vaccines against avian pasteurella multocida is imperative. inthis study, the genes encoding outer membrane protein h and a (omph and ompa) were cloned into theeukaryotic expression vector pcdna3.1(+) and the recombinant plasmids, namely dna vaccin...

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