background : toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. congenital toxoplasmosis is more important during pregnancy for the first time. in this study we expressed and purified p43 toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. methods : the recombinant pgemex-1 contained toxoplasma p43 codi...

In this study, we investigated the role and molecular mechanism of p43 and YS-1 (recombinant human p43 protein) in Dll4-Notch1 signaling pathway. Active, small interfering RNA and recombinant plasmid targeting of p43 protein were used to infect human umbilical vein endothelial cells (HUVECs). Three-dimensional sprouting model, endothelial cell migration assay, and sprouting and tube formation a...

BACKGROUND Toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. Congenital toxoplasmosis is more important during pregnancy for the first time. In this study we expressed and purified P43 Toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. METHODS The recombinant pGEMEX-1 contained Toxoplasma P43 coding...

The multifunctional cytokine p43 acts on endothelial and immune cells to control angiogenesis and inflammation. In this report, we describe an additional activity of p43 that specifically promotes fibroblast proliferation and wound repair. In skin wound regions from mice, tumor necrosis factor-alpha induced p43 expression and secretion from macrophages recruited to the site. p43 also promoted f...

p43, a glycoprotein of pea chloroplast (ct), acts as an accessory protein of pea chloroplast DNA polymerase. p43 binds to DNA, binds to ct-DNA polymerase and stimulates the ct-DNA polymerase activity. In the work presented here, the C-terminal domain of p43 (p22) has been overexpressed in E. coli. South Western analysis reveals that the recombinant p22 lacks in DNA binding activity. However, th...

BACKGROUND Mannanase is an enzyme that can catalyze random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans which are the key polymers in hemicellulose. It has been used in a number of different industrial applications including food, feed, pharmaceutical, pulp/paper industries, and second generation biofuel. To optimize the expression sys...

The maltose α-amylase AmyM from Bacillus stearothermophilus can be used for flour modification, baked goods preservation, and production. Here, we optimized the recombinant expression of in subtilis WB800 via several strategies. By screening optimal promoter, a double promoter combination (P43 PamyL) could improve level by 61.25%, compared with strong P43. Then, secretion efficiency over-expres...

OBJECTIVE Apoptosis is a key feature of advanced atherosclerotic plaques. Attraction signals such as p43 released from apoptotic cells play a crucial role in the timely removal of the apoptotic remnants by recruiting fresh phagocytes. Here, we sought to determine whether p43 may link apoptosis to inflammation and plaque progression. METHODS AND RESULTS RT-PCR and immunohistochemistry showed t...

An auxiliary factor of mammalian multi-aminoacyl-tRNA synthetases, p43, is thought to be a precursor of endothelial monocyte-activating polypeptide II (EMAP II) that triggers proinflammation in leukocytes and macrophages. In the present work, however, we have shown that p43 itself is specifically secreted from intact mammalian cells, while EMAP II is released only when the cells are disrupted. ...

BACKGROUND The visual display on night vision goggles (NVG's) is green and isochromatic (P22 phosphor). Future systems are expected to use a P43 phosphor which has a narrower visible spectrum and is yellowish green, while the P22 is deeper green. In transitioning to the P43, some NVG's may have P22 and P43 phosphors paired in the same NVG. The purpose of this study was to evaluate the suitabili...