the batch fermentation production of human growth hormone (hgh) was studied in a newrecombinant escherichia coli. the effect of different levels of dissolved oxygen and glucoseconcentration on cell density and substrate utilization were evaluated. the results indicated that theoptimum level of dissolved oxygen was 25% of air saturation. the specific growth rate reached amaximum for an optimal g...

background: in the recent years, there has been an increasing interest in secretory production of recombinant proteins, due to its various advantages compared with intracellular expression. signal peptides play a critical role in prosperous secretion of recombinant proteins. accordingly, different signal peptides have been assessed for their ability to produce secretory proteins by trial-and-er...

for high-throughput production of recombinant protein in escherichia coli ( e. coli ), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and gc content specially at n-terminal region should be considered. in the current study, the effect of decreasing the percentage of gc nucleotides and opt...

in order to study the periplasmic expression of human growth hormone (hgh) in escherichia coli, the related cdna was inserted in two expression plasmids carrying pelb signal peptide, one with lac bacterial promoter and the other with a bacteriophage t7-based promoter. the recombinant plasmids were moved to tg1 and bl21 strains of e. coli, respectively. to induce the expression systems, iptg and...

1. In adult humans with growth hormone deficiency, treatment with growth hormone has recently been shown to have major anabolic effects and to improve exercise performance. The cardiovascular effects of growth hormone in adults with growth hormone deficiency were examined in 24 patients treated with recombinant human growth hormone (0.07 units/kg at night) in a double-blind, placebo-controlled ...

human growth hormone (hgh) genomic sequence containing 5 exons and 4 introns was cloned in pcdna-3 and the constructed plasmid was subsequently used for transfection ofnlli-3t3 cell line using lipofection technique. expression of hgh in stably transfected cells was assayed using elisa. total rna was extracted from transfected cells and hgh cdna was amplified by rt-pcr using specific primers. th...

OBJECTIVES In laryngeal cancer, which comprises 25% of head and neck cancer, chemotherapy has come into prominence with the increase in organ-protective treatments. With such treatment, salvage surgery has increased following recurrence; the incidence of pharyngocutaneous fistula has also increased in both respiratory and digestive system surgery. We investigated the effects of recombinant huma...

BACKGROUND Human growth hormone (hGH) is a single-chain polypeptide that participates in a wide range of biological functions such as metabolism of proteins, carbohydrates and lipids as well as in growth, development and immunity. Growth hormone deficiency in human occurs both in children and adults. The routine treatment for this condition is administration of recombinant human growth hormone ...