background: non-dystrophic myotonias are a heterogeneous set of skeletal, muscular channelopathies, which have been associated with point mutations within sodium channel α-subunit (scn4a) gene. because exons 22 and 24 of scn4a gene are recognized as hot spots for this disease, the purpose of the study is to identify mutation in exons 22 and 24 of scn4a gene in iranian non-dystrophic myotonias p...

introduction the aim of this study was toinvestigate the sterilization potential of atmospheric pressure plasma jet (appj) and interactions of this technology with double-stranded dna using the polymerase chain reaction (pcr) and single-strand conformation polymorphism (sscp) techniques. materials and methods the plasma jet was produced through a high voltage sinusoidal power supplyusing a mixt...

background: the incidence rate of breast cancer has been dramatically increasing since the last decade in iran, and it is now one of the most common female malignant tumors. b?cell lymphoma 2 (bcl2) family is the most important regulator of apoptosis, and ?938c>a single nucleotide polymorphism (snp) of bcl2 gene promoter has been demonstrated to influence breast cancer susceptibility. in this r...

molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in livestock. the insulin like growth factor-i (igf-i) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. the objectives of the present study were the estimate the haplotype frequencies of the igf-i gene polymorphisms i...

The conversion of a nucleic acid from single strands to double strands is thought to involve slow nucleation followed by fast double-strand propagation. Here, for RNA double-strand propagation, we propose an atomic resolution reaction mechanism. This mechanism, called the stack-ratchet, is based on data-mining of three-dimensional structures and on available thermodynamic information. The stack...

familial hypercholesterolemia (fh) is an autosomal co-dominant disorder of lipid metabolism, caused by mutations in ldl receptor gene. the penetrance of fh is almost 100%, meaning that half of the offspring of affected parents born with disease. the patients are at risk of premature coronary heart disease (chd). there is no report about the molecular basis of fh in iran. identification of mutat...

The rate of appearance of duplex DNA renaturation, measured with single strand specific nuclease, deviates significantly from a second order reaction. Measurements reported in paper I of this series indicate an inhibition in the rate of reassociation of single strand tails on partially reassociated molecules by a factor of at least two. Equations are derived that describe the observed form of r...

Background: Endometriosis is a complex disease that profoundly affects the quality of life in many women. This disease affects roughly one in ten women of reproductive age. Endometriosis induces a variable amount of inflammatory reaction in pelvic environment. An active immune system needs to recognize these inflammatory agents. Rapid innate immune system defenses against infections involve the...

background and aims. this study aimed to investigate release of nickel ion from three types of nickel-titanium-based wires in the as-received state and after immersion in a simulated oral environment. materials and methods. forty specimens from each of the single-strand niti (rematitan “lite”), multi-strand niti (speed supercable) and copper niti (damon copper niti) were selected. twenty specim...