it is difficult to distinguish between clinically significant slowly-growing, non-pigmented mycobacteria, notably to separate m. aviumand m. intracellulare from one another and from m. scrofulaceum strains. the purpose of this study was to evaluate the extent to which 16s rrna sequencing could be used to highlight the taxonomic relationships of the mycobacterial strains, which are difficult to ...

tarkhineh is one of the traditional foods in iran and a rich source of probiotic bacteria. the objective of this study was to identify the probiotic bacteria isolated from tarkhineh using 16s rrna gene sequencing and molecular typing with repetitive extragenic palindromic–pcr (rep-pcr). in total, 20 different bacteria were isolated from traditional dairy products and tarkhineh. molecular identi...

the intestinal bacterial diversity of stichopus japonicus was investigated using 16s ribosomal rna gene (rdna) clone library and polymerase chain reaction/denaturing gradient gel electrophoresis (pcr-dgge). the clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (otus) with sequence similarity ranging from 88 to 100%. the cov...

The 16S rDNA polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and sequencing method has been demonstrated to be valuable in detecting pathogens in the blood of patients suffering from fever or neutropenia. However, its use in the diagnosis of neonatal late‑onset septicemia (LOS) has not yet been reported. The aim of the present study was to investigate the efficien...

The identification of Mycobacteria in the species level has great medical importance. Biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. This research aimed to the comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria in patients suspected to tuberculosis in Golestan province which is the ...

the aim of this study was to evaluate the performances of methods such as sequencing of the internal transcribed spacer (its) and 26s (d1/d2) regions of ribosomal dna, rflp analysis of the its region and commercial biochemical test kit for the identification of the yeasts isolated during spontaneous fermentation of fresh crushed pineapple juice. the experiments were conducted in thailand and au...

Microbial translocation (MT) from the gut is implicated in driving immune activation, increasing morbidity and mortality in HIV. We used bacterial 16S rDNA PCR, Sanger sequencing, and high-throughput sequencing to identify microbial DNA in the bloodstream of HIV-infected children in London, United Kingdom. Blood samples were collected from sequential children attending the HIV clinic at Great O...

Sequencing of the gene coding for 16S rRNA (16S rDNA) is a well-established method used to identify bacteria, particularly mycobacteria. Unique sequences allow identification of a particular genus and species. If more than one 16S rDNA is present on one mycobacterial genome, their sequences are assumed to be strictly or almost identical. We have isolated a slowly growing Mycobacterium strain, "...

Wound healing is a complex sequence of events consisting of regeneration and repair. It has been proposed that bacteria present in wounds play a major role in delayed wound healing. The main objectives of this study are to identify the bacterial species present in wounds and to evaluate and compare the results obtained using conventional biochemical analysis and 16S rDNA sequencing. Wound swabs...

PREMISE OF THE STUDY Just as Sanger sequencing did more than 20 years ago, next-generation sequencing (NGS) is poised to revolutionize plant systematics. By combining multiplexing approaches with NGS throughput, systematists may no longer need to choose between more taxa or more characters. Here we describe a genome skimming (shallow sequencing) approach for plant systematics. METHODS Through...