AIM To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental osteoarthrosis. METHODS Twenty dogs aged 2-8 years were divided in research (aged 2 years, induction of osteoarthrosis - IOA) and intact group. Intact group included three subgroups (aged 2, 5 and 8 years). Samples of cartilage after araldite satur...

We report a simple method to study the elemental content in cultured human adherent cells by electron probe X-ray microanalysis with scanning electron microscopy. Cells were adapted to grow on polycarbonate tissue culture cell inserts, washed with distilled water, plunge-frozen with liquid nitrogen and freeze-dried. Unstained, freeze-dried cultured cells were visualized in the secondary and bac...

The analytical electron microscope (AEM) uses a high energy (>100 kV) beam of electrons to generate a range of signals from a thin foil sample as shown in figure la [1,2]. Various detectors are configured in the AEM to pick up most of the generated signals (fig. lb). Microanalysis is usually performed using the characteristic x-ray signal, detected by an energy dispersive spectrometer (EDS) alt...

BACKGROUND Reconstructive hair fibers are an innovative tool in cosmetic dermatology based on electrostatic adherent powder which bonds to the hair shaft and can disguise hair loss in men and women. AIM This study aims to analyze and compare five different brands of organic hair fibers. MATERIALS AND METHODS A scanning electron microscope (SEM) was used to obtain amplified images of samples...

introduction: the present study evaluated the element distribution in completely set calcium-enriched mixture (cem) cement after application of 35% carbamide peroxide, 40% hydrogen peroxide and sodium perborate as commercial bleaching agents using an energy-dispersive x-ray microanalysis (edx) system. the surface structure was also observed using the scanning electron microscope (sem). methods ...

X-ray microanalysis has the potential to extend beyond high resolution element mapping of the obvious and not-so-obvious. The real power of X-ray microanalysis is the speed and accuracy with which it can enable true discovery – uncovering the unexpected chemical phase(s) that element-based image analysis alone cannot detect given the limitations of distilling massive amounts of spectral data in...