background: it has been reported that secreted frizzled-related protein-4 known as an antagonist of wnt signaling pathway plays a role in luteinization process of rodent granulosa cells. the purpose of this study was twofold: 1) to determine whether recombinant human secreted frizzled-related protein-4 (rhsfrp-4) could directly induce terminal differentiation of rat granulosa cells (gcs) and 2)...

the association of secreted frizzled related protein type 4 (sfrp4) as an antagonist of wnt mole-cules in apoptotic events has been reported previously. moreover, its increased expression has been reported in the ovary of women with polycystic ovary (pco). we have demonstrated in-creased sfrp4 in pco-induced rat ovary related to an increased number of apoptotic follicles showing nuclear ?cateni...

The association of secreted frizzled related protein type 4 (Sfrp4) as an antagonist of Wnt mole-cules in apoptotic events has been reported previously. Moreover, its increased expression has been reported in the ovary of women with polycystic ovary (PCO). We have demonstrated in-creased Sfrp4 in PCO-induced rat ovary related to an increased number of apoptotic follicles showing nuclear ?cateni...

objective(s): pesticides has wide range of infertility in female reproductive. this study was done to evaluate the effect of movento pesticide on development of granulosa cells and ovarian follicles and foxo1 and vnn1 gene expression in balb/c mice. materials and methods: in this study 40 healthy balb/c mice 5-6 weeks age were used. animals were randomly allocated into four groups. control (wit...

It is well established that rat ovarian granulosa cells produce tissue plasminogen activator (tPA). The synthesis and secretion of the enzyme are induced by gonadotropins, and correlate well with the time of follicular rupture in vivo. We have found that in contrast, mouse granulosa cells produce a different form of plasminogen activator, the urokinase-type (uPA). As with tPA synthesis in the r...

Prohibitin (Phb1) is a highly conserved mitochondrial protein that is associated with granulosa cell differentiation, atresia, and luteolysis. Although prohibitin has been implicated in the suppression of apoptosis in mammalian cells, its specific role in programmed cell death in granulosa cells is unknown. In the present study, we examined the role of prohibitin in mediating staurosporine (STS...

Ovarian follicle atresia is thought to be induced through apoptosis of granulosa cells. This study was designed to investigate the possible involvement of nitric oxide (NO) in granulosa cell apoptosis. In immature rat ovaries obtained 48 h after pregnant mare serum gonadotropin administration, immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTPbiotin nick end labeling (...

Apolipoprotein E (apoE) is synthesized by the liver and many peripheral cells. Rat ovarian granulosa cells synthesize and secrete apoE, and this apoE production is increased by agents that increase cellular cAMP. In these studies of granulosa cell apoE synthesis we have examined the effect of agents that stimulate various cell kinases, including protein kinases A, G, and C. The cell content of ...

The National Toxicology Program (NTP) classification system for rat and mouse ovarian tumors is presented. The classification system is based on previous classification systems and on a review of all the primary ovarian tumors from the archives of the National Cancer Institute (NCI) and NTP Carcinogenesis Testing Programs. The relative frequency and principal diagnostic features of 204 ovarian ...