At the present time there is no satisfactory procedure for estimating the degree of gram positivity although the need for a quantitative method is widely recognized. Recently Kennedy and Barbaro (1953) reported an accurate and reproducible method for determining quantitatively the adsorption of crytl violet by bacteria. In addition, the effect of the species and strain of the organism used, the...

background: the wilms tumor 1 (wt1) gene is originally defined as a tumor suppressor gene and a transcription factor that overexpressed in leukemic cells. it is highly expressed in more than 80% of acute myeloid leukemia (aml) patients, both in bone marrow (bm) and in peripheral blood (pb), and it is used as a powerful and independent marker of minimal residual disease (mrd);we have determined ...

vaccines is considered as a critical point and in-process of quality control (ipqc) test of vaccine production. rapid tests, like real time pcr, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. in the current study, a real-time quantitative reverse transcription-polymerase chain reaction (...