ABSTRACT Traditional methods of quantifying Pythium spp. rely on the use of selective media and dilution plating. However, high variability is inherent in this type of enumeration and counts may not be representative of the pathogenic population of Pythium spp. Variable regions of the internal transcribed spacer of the rDNA were used to design species-specific primers for detection and quantifi...

abstract the pythium spp. are known as the principle agents of seedling blight, damping-off and root rot of cucurbits. this study was performed for determination of pythium species involved in this diseases as well study their pathogenecity on different cucurbits. the numerous samples were taken at cultivation seasons 2006- 2007 from the fields of cucumber, tomato, egg plant, muskmelon, cucurbi...

Diagnosis of Pythium keratitis is problematic due to the difficulty in obtaining a culture report resulting in unnecessarily prolonged usage of antimicrobial medication due to misdiagnosis. This study evaluated and compared nested PCR technique with culture and immunoperoxidase staining assays of Pythium insidiosum in paraffin-embedded corneal tissues from patients with suspected fungal keratit...

We report the first case of pythiosis from Africa in an 8-month-old dog with a chronic and ulcerative cutaneous lesion. The etiologic agent belonged to the genus Pythium. Phylogenetic analysis placed the isolate in a sister group to the other P. insidiosum strains. However, the isolate may belong to a new Pythium species.

during 2000-2001, soil pythium species were studied in fars province. soil samples werecollected from various parts of the province (abadeh, bajgah, bayza, borazjan, darab, estahban, fasa,khafr, sepidan, shiraz, moharloo, and zarghan). isolates were recovered from soil by baiting. specieswere identified based on morphological characters of sexual and asexual organs, growth rate at differenttemp...

Five strains of Trichoderma with known biocontrol activities were assessed for their effect upon pea growth and their antagonistic activity against large Pythium ultimum inocula. The effect of Trichoderma inocula upon the indigenous soil microflora and soil enzyme activities in the presence and absence of Pythium is assessed. In the absence of Pythium, Trichoderma strain N47 significantly incre...

This experiment aims at establishing the ability of the endomycorrhizal fungus Glomus intraradices to suppress the plant pathogen Pythium ultimum in cherry tomato. A study published in Canadian Journal of Plant Pathology in 1994 showed that Glomus intraradices suppresses Pythium ultimum in Tagetes patula (french marigold) (St-Arnaud, Fortin, Caron, & Hamel, 1994). Pythium ultimum and other Pyth...

A description is given of Pythium litorale sp. nov., a new species from reed stands in Germany. Pythium litorale was among the most abundant species when the oomycete community of littoral soils of Lake Constance was studied. It was consistently isolated from flooded as well as from drier reed sites. The species is characterized by subglobose, papillate and internally proliferating sporangia, g...