Pyronine at non-toxic concentrations greatly reduced the yield of encephalomyocarditis virus produced by Krebs ascites cells. The inhibition was greatest when pyronine was added 2 hr after infection and was less when the dye was added later. It also decreased when the dye was added shortly before infection. It could be reversed by removing pyronine from infected cells less than 2 hr after treat...

A mitochondria-targetable fluorescence probe, methyl(4-hydroxyphenyl)amino-substituted pyronin (1), was exploited, which could highly selectively sense peroxynitrite (ONOO(-)) within seconds.

The cation of methyl green carries two positive charges, that of malachite green only one; but the two dyes behave towards tissue-constituents in almost exactly the same way. These dyes are not specific for chromatin. They colour certain objects that are devoid of DNA, even when they are used in very dilute solution. The granules of cells called Kornchenzellen in the connective tissue of the co...

Pyronin Y (PY) has a strong resonance fluorescence in sulfuric acid medium. The characteristics of the resonance fluorescence spectra and the factors affecting the spectra were studied. A catalytic resonance fluorometry method for the determination of hydroquinone was proposed based on the catalytic effect of hydroquinone on the oxidation of PY by potassium bromate. The oxidation of PY resulted...

Following the injection of various antigenic and non-antigenic materials into the foot-pads of rabbits, the draining (popliteal) lymph nodes were removed on successive days and studied histologically, chemically, and serologically. On the 2nd day after injection of antigen, nucleoli and cytoplasmic granules and crescents stained with pyronine began to appear. They were found first in somewhat a...

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A new histochemical technique for morphological studies of mononuclear cells and granulocytes, based on fluorescent staining with methyl green, pyronine Y, and stilbene-isothiocyanato disulfonic acid (MPS stain) is described. The method was applied to mononuclear cells isolated from the blood of normal human subjects by Ficoll-Isopaque density centrifugation. Three cell populations were disting...

The primary spermatocytes of Drosophila virilis have been shown to undergo an approximate 300-fold increase in volume and to possess an abundance of ribo somes in the cytoplasm (Ito 1960). These ribosomes are probably involved in the biosynthesis of the various materials needed for this tremendous volumetric increase. Olivieri and Olivieri (1965) have demonstrated that RNA synthesis occurs in s...