Although pyrimethamine (Tindurin™) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. We design this study to evaluate its anti-tumoral effect on a model of cell line. Methods: The cytotoxic influence of Pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. The po...

background: point mutations in the genes, pfcrt and pfdhfr , have been identified elsewhere as strongly associated with re­sistance to chloroquine (cq) and pyrimethamine, respectively.  methods: using a restriction digestion method, we report the frequency of two important mutations, t76 and asn108, re­spon­sible for conferring resistance to chloroquine and pyrimethamine, respectively, in patie...

although pyrimethamine (tindurin™) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. we design this study to evaluate its anti-tumoral effect on a model of cell line. methods: the cytotoxic influence of pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. the po...

Pyrimethamine is an antimalarial drug which possesses schizonticidal activity in both tissue and blood phases. Though pyrimethamine is known to inhibit folic acid metabolism of malarial parasites by binding with folio acid reductase (6), the cytological effect on the parasites is not completely understood. To our knowledge, there has been no study until now on the morphological effects of pyrim...

The unresponsiveness of metastatic melanoma to conventional chemotherapeutic and biological agents is largely due to the development of resistance to apoptosis. Pyrimethamine belongs to the group of antifolate drugs, and in addition to antiprotozoan effects, it exerts a strong proapoptotic activity, which we recently characterized in human T lymphocytes. However, no data regarding pyrimethamine...

We describe the isolation and the sequence of the gene for the bifunctional enzyme dihydrofolate reductase-thymidylate synthase (DHFR-TS; EC 1.5.1.3 and EC 2.1.1.45, respectively) from two pyrimethamine-resistant clones of Plasmodium falciparum, HB3 and 7G8. We have also derived the sequence of the DHFR portion of the gene, by amplification using polymerase chain reaction, for the pyrimethamine...

Three antimalarial drugs, artesunate, pyrimethamine, and pamaquine, were evaluated for their growth-inhibitory effects against Babesia equi and Babesia caballi in in vitro culture. B. equi was more resistant to pyrimethamine than B. caballi. B. equi was also found to be more sensitive to artesunate and pamaquine than B. caballi. Of the three compounds, pyrimethamine gave the most promise for in...

CONTEXT In malaria-endemic regions, strategies to control malaria during pregnancy rely on case management of malaria illness and anemia, and preventive measures such as insecticide-treated nets and intermittent preventive therapy (IPT). OBJECTIVE To determine the effect of increasing resistance to sulfadoxine-pyrimethamine on the efficacy of IPT during pregnancy in Africa. DATA SOURCES AND...