Abstract Pyrazinamide (PZA), a medication for tuberculosis, has high aqueous solubility and low permeability, undergoes extensive liver metabolism, exhibits toxicity through its metabolites. To avoid this, PZA in lipid core-shell nanoarchitectonics been formulated to target lymphatic uptake provide metabolic stability the incorporated drug. The UPLC-MS/MS method reliable vitro quantitative anal...

OBJECTIVES To compare the performance of the BacT/ALERT PZA kit (BioMerieux, Marcy l'Etoile, France) with the radiometric BACTEC 460TB PZA test (Becton-Dickinson method) for testing Mycobacterium tuberculosis susceptibility to pyrazinamide. METHODS A total of 50 M. tuberculosis strains were tested. Thirty of these strains had been previously considered pyrazinamide-susceptible and 20 pyrazina...

We report draft genome sequences of two pyrazinamide (PZA)-resistant isolates, Mycobacterium tuberculosis 13-4152 and 13-2459. Isolate 13-4152 is PZA resistant, though it lacks mutations in known genes of PZA resistance. The comparative analysis of these genomes with those stored in GenBank revealed unique mutations, which may elucidate new mechanisms of PZA resistance.

5-[18F]F-pyrazinamide (5-[18F]F-PZA), a radiotracer analog of the first-line tuberculosis drug pyrazinamide (PZA), was employed to determine the biodistribution of PZA using PET imaging and ex vivo analysis. 5-[18F]F-PZA was synthesized in 60 min using a halide exchange reaction. The overall decay-corrected yield of the reaction was 25% and average specific activity was 2.6 × 106 kBq (70 mCi)/μ...

Background: Pyrazinamide is one of the most important first-line medications for treatment tuberculosis and an alternative intake MDR-TB XDR-TB patients. Objectives: The purpose this study was to evaluate resistance pyrazinamide in isolates resistant Mycobacterium drug patients city Isfahan. Methods: In study, susceptibility test performed with using proportion method PZA assay on 47 tuberculos...

Fifty isolates of Mycobacterium tuberculosis complex were tested for susceptibility to pyrazinamide (PZA) by the ESP (Trek Diagnostic Systems, Westlake, Ohio) and BACTEC (BD Biosciences, Sparks, Md.) test systems. Initial results showed concordance for 48 of the isolates. On retest, the two discordant isolates were resolved in favor of the ESP system. The ESP Myco susceptibility test system gen...

Electronic and optical properties of 2D models of graphene, boron nitride (BN), silicene, SiC, and phosphorene functionalized with pyrazinamide (PZA), a front line antitubercular chemotherapeutic, are investigated using cluster models and density functional theory with van der Waals dispersion corrections and including solvent effects. PZA favors covalent functionalization onto silicene and SiC...

Mycobacterium bovis is best identified by screening those isolates of the Mycobacterium tuberculosis complex that have any pyrazinamide (PZA) resistance, using a confirmatory test such as spoligotyping, biochemical testing, or genomic deletion analysis. The sensitivity for detection of M. bovis is lowered to 82% when only PZA-monoresistant isolates are screened.

BACKGROUND & OBJECTIVES Pyrazinamide is an essential component of first line anti-tuberculosis regimen as well as most of the second line regimens. This drug has a unique sterilizing activity against Mycobacterium tuberculosis. Its unique role in tuberculosis treatment has lead to the search and development of its structural analogues. One such analogue is 5-chloro-pyrazinamide (5-Cl-PZA) that ...

The correlation between resistance to pyrazinamide (PZA) and resistance to other first-line antituberculosis drugs was investigated in 395 Mycobacterium tuberculosis strains isolated from clinical specimens, representing 14% of the overall number of M. tuberculosis isolates obtained between 2003 and 2008 at the laboratory of a referral university hospital for tuberculosis. A high correlation wa...