BACKGROUND The presence of "anti-DNA antibodies in abnormal titres" is a well established criterion for SLE classification, but there is no agreement on the performance of this test. OBJECTIVE To study the correlation between clinical findings and five different solid and solution phase anti-DNA antibody assays. METHODS 158 consecutively collected ANA positive sera were studied in a double ...

Complexes of Cu(II), Ni(II) and Mn(II) containing a novel homotrinuclear Schiff base metal complexes were synthesized by using 2,3-pentanedione, p-phenylenediamine and 2-aminobenzaldehyde. The complexes of the type [LM 3 Cl 6 ]. The characterization and nature of bonding of the trinuclear complexes have been deduced from elemental analysis, molar conductivity, FT-IR, UV-visible, magnetic and EP...

PHRED is the most frequently used base caller algorithm in genome projects. An interesting point on PHRED utilization is the fact that a low score on some base may not actually correspond to a miscalling on that base, but it may stand for a putative error on the region around this base. In order to evaluate the efficiency of PHRED on base calling and base quality assigning, we have sequenced pU...

SOURCE/DESCRIPTION; A 2.0 kb Mspl fragment from cosmid YNH24 isolated by HBV-2 oligonucleotide (GGAGTTGGGGGAGGAG) (1) was subcloned into the AccI site of pUC18. POLYMORPHISM: Mspl identifies a >30 allele VNTR polymorphism with bands between 1.0-5.0 kb. Taql, Bglll, Pvull, PstI and BamHI also detect the same polymorphism.

Analysis of DNA samples for restriction fragment length polymorphisms requires correct recognition of the normal restriction sites. Some restriction endonucleases have been shown to exhibit altered cut site specificity (star activity) under certain reaction conditions (1, 2). We report here the results of experiments demonstrating Hinfi star activity. Two ngs of pUC18 DNA or SV40 DNA were diges...

The plasmid pUC18 contains a lacZ alpha-complementation gene that codes for a small peptide that can complement the delta M15 mutation of the Escherichia coli lacZ (beta-galactosidase) gene, converting bacteria carrying that mutated gene from the lacZ- to the lacZ+ phenotype. This plasmid was used in experiments designed to study mutagenesis by human neutrophils. E coli carrying pUC18 were incu...

Source/Description: A 1.6 Kbp fragment of the cDNA clone for the human ethanol inducible cytochrome P450 gene cloned in pUC18 at the EcoRI restriction site (Song and Gonzalez, 1986). Polymorphism: Xmnl detects a two allele polymorphism with bands at 16 kb (Al), 11.4 and 4.6 kb (A2). Frequency: A total of 37 unrelated individuals of Caucasian origin were screened: Al 0.81; A2 0.19.

We have investigated the phase behaviour of pUC18 plasmid solutions with phase separation experiments and polarized light microscopy. Furthermore, the configuration of the superhelix is monitored with small-angle neutron scattering. The phase diagram is interpreted with liquid crystal theory including the effects of charge, orientation entropy, excluded volume, as well as the elastic, entropic ...