in this study, mutant forms of bacillus thuringiensis spp. israelensis (h14) were produced. these mutants were identified when the cells were cultured on chloramphenicol plates and stained with crystal violet. protoplasts of the mutants were isolated by enzymatic digestion (lysozyme) of the cell walls at the presence of an osmotic stabilizer. the protoplasts were induced to fuse to each other i...

Liposomes entrapping fluorescein diacetate were fused with protoplasts of Datura innoxia Mill by employing polyethylene glycol (PEG) as the fusogen. Factors that influence liposome-protoplast fusion were optimized as a function of PEG-concentration and incubation duration, liposome composition and surface charge and liposome:protoplast ratio. Phosphatidylcholine-liposomes were found ideal for t...

Filamentous fungi Trichoderma spp. are efficient hyper-producers of industrially important enzymes. Xylanases are essential hemicellulases having vast ranges of applications in the various industrial sectors. In this study, the protoplast fusion of two T. virens and </span...

The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breeding. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resis...

Departme Tusa, N., J.W. Grosser, and F.G. Gmitter, Jr. 1990. Plant regeneration of ‘Valencia’ sweet orange, ‘Femminello’ lemon, and the interspecific somatic hybrid following protoplast fusion. J. Amer. Soc. Hort. Sci. 115:1043– 1046. Vardi, A., P. Arzee-Gonen, A. Frydman-Shani, and S. Bleichman. 1989. Protoplast fusion mediated transfer of organelles from Microcitrus and Citrus and regeneratio...

The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formati...

Brassica coenospecies has been bestowed with a large collection of wild and weedy relatives, which could act as donors of genes controlling agronomically important traits like disease resistance, yield attributes, modified fatty acid composition in the seed etc. Additionally, the cytoplasm of these species could be the sources for developing alloplasmic male sterility systems that are important...

Cosynthesis in mixed culture and protoplast fusion of non-producing mutants of Streptomyces hygroscopicus which may produce biosynthetic intermediates of bialaphos (AMPBA) were studied. Non-producing mutants were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) treatment, and six stable non-producing mutants were used for cosynthesis and protoplast fusion studies. The bio...