نتایج جستجو برای: protoplast cell

تعداد نتایج: 1684812  

Journal: :چغندرقند 0
بهروز احسانی مقدم عضو هیئت علمی موسسه تحقیقات چغندرقند نسرین یاوری کارشناس موسسه تحقیقات چغندرقند

the most important applications of sugar beet protoplast culture are production of transgenic plants and somatic hybridization. fortunately, to date many obstacles which were involved with sugar beet protoplast culture, have successfully been removed in iran. the production of whole plant via protoplast culture as the most final step towards using this technique is the base of this investigatio...

The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...

Journal: :Journal of general microbiology 1981
R H Baltz P Matsushima

Protoplasts from four different species of Streptomyces regenerated cells efficiently in hypertonic soft agar medium overlaid on partially dehydrated regeneration medium. The efficiencies of regeneration were strongly dependent upon the incubation temperatures for cell growth and for protoplast regeneration. Cell growth temperatures (before protoplast formation) required for efficient protoplas...

Journal: :journal of crop protection 2013
sohrab moradi forough sanjarian naser safaie amir mousavi gholam reza bakhshi khaniki

one of the important technical obstacles in the study of many filamentous fungi is the development of efficient transformation system. transformation of filamentous fungi is difficult because they have a cell wall and for most frequently used approaches preparing of protoplast is necessary. protoplast preparation is batch-dependent and often frustrating work. in this study, a modified method wa...

2014
Xiaoqin Wang Meiyan Qi Jingyun Li Zhongzhong Ji Yong Hu Fang Bao Ramamurthy Mahalingam Yikun He

The moss Physcomitrella patens is an ideal model plant to study plant developmental processes. To better understand the mechanism of protoplast regeneration, a phosphoproteome analysis was performed. Protoplasts were prepared from protonemata. By 4 d of protoplast regeneration, the first cell divisions had ensued. Through a highly selective titanium dioxide (TiO2)-based phosphopeptide enrichmen...

Journal: :Plant Cell Tissue and Organ Culture 2023

Abstract In the present study, a high yield of isolated protoplasts from agronomically important crop Fagopyrum esculentum was obtained by applying mixture cellulase, pectolyase, and driselase. We demonstrated that morphogenic callus-derived 1 × 10 6 per g fresh tissue. For hypocotyls used as protoplast source, number released cells twice lower. The protoplasts, embedded in an agarose matrix cu...

Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...

Journal: :journal of sciences islamic republic of iran 0

the use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. fifty to one hundred µm silver thiosulfate (sts) produced a larger leaf area as a source of plant material for protoplast isolatio...

Journal: :Plant physiology 1979
B E Gómez-Lepe O Lee-Stadelmann J P Palta E J Stadelmann

Effects of octylguanidine (OG) were studied on the permeability of cells of the adaxial epidermis of Allium cepa bulb scales to water and methyl urea and on the protoplast surface. Interference of OG with the Ca(2+) and Al(3+) action on the cell surface was also investigated.Permeability of the cell membrane for water and methyl urea increased nearly three times in presence of OG. The effect of...

2015
Terence W.-Y. Tiew Michael B. Sheahan Ray J. Rose

The ability to induce Arabidopsis protoplasts to dedifferentiate and divide provides a convenient system to analyze organelle dynamics in plant cells acquiring totipotency. Using peroxisome-targeted fluorescent proteins, we show that during protoplast culture, peroxisomes undergo massive proliferation and disperse uniformly around the cell before cell division. Peroxisome dispersion is influenc...

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