BACKGROUND AND AIMS Cymbidium mastersii is an epiphytic orchid distributed mainly in Northeast India. Owing to its high commercial value in the floricultural industry, natural populations are under threat from over-exploitation. Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for ...

Gametophytic selection was examined as a breeding tool in developing Phalaenopsis hybrids that are more cool or warm temperature tolerant. Two hybrid Phalaenopsis, P. (Taisoco Windian × Sogo Yukidian) by P. hybrid unknown, were reciprocally crosspollinated and exposed to 14°C/9°C for 7 days as a cold pollination treatment. Plants were pollinated again and exposed to 30°C/25°C for 3 days for the...

Orchid mycorrhizal protocorms and roots are heterogeneous structures composed of different plant cell-types, where cells colonized by intracellular fungal coils (the pelotons) are close to non-colonized plant cells. Moreover, the fungal coils undergo rapid turnover inside the colonized cells, so that plant cells containing coils at different developmental stages can be observed in the same tiss...

BACKGROUND AND AIMS The maintenance of species boundaries in sympatric populations of closely related species requires some kind of reproductive isolation that limits gene flow among species and/or prevents the production of viable progeny. Because in orchids mycorrhizal fungi are needed for seed germination and subsequent seedling establishment, orchid-mycorrhizal associations may be involved ...

Dendrobium officinale Kimu et Migo has increased many researchers' interest for its high medical and horticultural values and the molecular mechanism of its protocorm development remains unclear. In this study, 19 genes from 26 most stably expressed genes in whole transcriptome of protocorms and 5 housekeeping genes were used as candidate reference genes and screened with 4 application software...

We investigated the appropriate growth conditions of shoot and root through in vitro propagation of orchid (Dendrobium sonia) using different gelling agents. Protocorm Like Bodies (PLBs) of orchid were initiated earlier in vitro and one month old protocorms were used for this study. Protocorms were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of starche...

The present study describes the encapsulation of protocorm of Cymbidium bicolor Lindl. from 60 days-old in seed cultures for short- term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were ob- tained through a combination of 3% sodium alginate...

Abstract The objective of this study was to evaluate the efficiency cryoprotective solution (PVS2) combined with phloroglucinol for cryopreservation seeds two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds had 91.03% initial viability 91.99% germination. treatment PVS2 at 0 °C 1% 60 min returned 93.79% 91.01% germination after recovery from LN, consequently resulting in faster...

in this study, in vitro non-symbiotic germination of self pollinated seeds, protocorms survival percentage and protocorms growth of phalaenopsis ‘kyoto’ were investigated. for this purpose, five different modified media include vasin and went (vw), murashing and skoog (ms), ½ ms, knudson c (kc), fast that supplemented with two g/l peptone, 15 percent sterile coconut water or two g/l peptone tog...

the present study describes the encapsulation of protocorm of cymbidium bicolor lindl. from 60 days-old in seed cultures for short- term conservation and propagation. various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. the ideal beads were ob- tained through a combination of 3% sodium alginate...