the effects of pasteurization, storage time and temperature on proteolysis of milk were evaluated. two replicates of each of raw and pasteurized milk were assigned to three temperatures (5, 16 and 25 ?c) and 3 storage periods (1, 2 and 3 day) in a 2×3×3 full factorial design. for all the samples, ph and indices of proteolysis were determined. the effect of all the three main factors (pasteuriza...

Background and Objectives: In this study, the proteolysis and lipolysis of Poosti cheese produced from raw sheep milk in mountainous eastern regions of Iran were investigated during 90 days of ripening. Materials and Methods: Sodium dodecyl sulfate polyacrylamide gel electrophoresis for proteolysis (SDS-PAGE) and gas chromatography (GC) for free fatty acids (FFAs) were applied to investigate th...

Pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and ADP to yield ATP and Pyruvate. Geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. Given that limited proteolysis experiments can be successfully used to probe conformational features of p...

pyruvate kinase is a key enzyme in glycolytic pathway that catalyzes the transphosphorylation between phosphoenolpyruvate and adp to yield atp and pyruvate. geobacillus stearothermophillus has a stable pyruvate kinase with determined crystal structure that composed of four separate domains. given that limited proteolysis experiments can be successfully used to probe conformational features of p...

background and objectives: in this study, the proteolysis and lipolysis of poosti cheese produced from raw sheep milk in mountainous eastern regions of iran were investigated during 90 days of ripening. materials and methods: sodium dodecyl sulfate polyacrylamide gel electrophoresis for proteolysis (sds-page) and gas chromatography (gc) for free fatty acids (ffas) were applied to investigate th...

The most widespread method applied for standardization of milk protein in cheese production isultrafiltration. In the current study, pasteurized, unhomogenized whole milk was ultrafiltered with APVultrafiltration system, 51 spiral wound, polysulfone membrane with 20000 Da molecular weight cut off (DDS,Nakskov, Denmark) at 50 ˚C, such that the pressure difference between the two sides of membran...

Proteolysis of bovine serum albumin by trypsin.

the objective of this study was to determine the effect of co2 treatment on proteolysis, lipolysis and quality characteristics of refrigerated raw milk. four treatments of raw milk were selected (controlled milk (milk without any special treatment) and milks treated with co2 until ph = 6.4, 6.2, 6). all experiments were triplicate. samples were stored at 4°c for 10 days and were analyzed on 1, ...

the effects of allium sativum on yogurt formation and subsequent storage (4°c, up to 28 days) on proteolysis, microbial activity, and inhibition of α-amylase, α-glucosidase and angiotensin-i converting enzymes were investigated in vitro. allium sativum yogurt showed higher rates of ph reduction and increment of titrable acid than control during the incubation at 41°c. highest proteolysis was ob...

Four different model infant formulae were compared under simulated conditions of the gastrointestinal tract using an in vitro dynamic digestion protocol. These based on whey protein isolate, isolate + lactoferrin, long chain triacylglycerols or medium triacylglycerols. In each four cases, influence and lipid composition their subsequent structural disintegration, proteolysis lipolysis during wa...