The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K sta...

the interaction of copper oxide nanoparticles and copper ions and proteinase k from tritirachium album limber has been investigated employing uv spectroscopy and kinetics measurements. the aim of this study was to evaluate the effect of nanoparticles of cuo for proteinase k. in this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase k stabilit...

   The interaction of Fe2O3, Fe3O4 and SiO2 nanoparticles with proteinase K activity was investigated using UV–vis spectroscopy. Proteinase K EC (3.4.21.14) is a member of serine protease family, which is produced from fungus Tritirachum album Limber.The effects of nanoparticles on proteinase...

In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treat...

Mitochondrial cholesterol 27-hydroxylase (CYP27A1) plays an important role in the maintenance of intracellular cholesterol homeostasis. Cholesterol delivery to the mitochondrial inner membrane is believed to be a rate-limiting step for the "acidic" pathway of bile acid synthesis. This work reports that proteinase K treatment of mitochondria markedly increases CYP27A1 specific activity. With end...

The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K stabilit...

in diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. if not inactivated properly, cellular and/or environmental dna nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in pcr experiments. when crude cell extracts, proteinase k–treat...

The capacity for self-polymerization and shape of the tubulin polymers assembled after digestion with trypsin, Pronase, chymotrypsin, subtilisin, Staphylococcus aureus proteinase V8 and proteinase K were investigated. Digestion with trypsin, Pronase or chymotrypsin resulted in a decrease in the ability of tubulin for self-assembly, whereas limited proteolysis with subtilisin, S. aureus proteina...

Three DNA extraction methods were evaluated in this study: proteinase K followed by phenol-chloroform; a plant proteinase (E6870) followed by phenol-chloroform; and boiling of leptospires in 0.1 mM Tris, pH 7.0 for 10 min at 100 degrees C, with no phenol treatment. Every strain treated with proteinase K or E6870 afforded positive polymerase chain reaction (PCR) reaction. On the other hand, from...