background: dna immunization with plasmid dna encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. objective: to evaluate the use of a dna immunization strategy for protection against anthrax, a plasmid was constructed. methods: the partialsequence of protective antigen of bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...

in the last decades researchers had focused on developing a vaccine against tick based on protective antigen. recombinant vaccines based on concealed antigen from boophilus microplus have been developed in australia and cuba by the name of tickgard and gavac (de la fuente and kocan, 2006). further studies on this antigen have shown some extent of protection against other species (de vos et al.,...

In the last decades researchers had focused on developing a vaccine against tick based on protective antigen. Recombinant vaccines based on concealed antigen from Boophilus microplus have been developed in Australia and Cuba by the name of TICKGARD and GAVAC (De La Fuente and Kocan, 2006). Further studies on this antigen have shown some extent of protection against other species (De Vos et al.,...

Anthrax toxin consists of three proteins, protective antigen, lethal factor, and edema factor. Protective antigen translocates lethal factor and edema factor to the cytosol of mammalian cells. The amino-termini of lethal factor and edema factor have several homologous stretches. These regions are presumably involved in binding to protective antigen. In the present study we have determined the r...

Protective antigen (PA), a subunit of anthrax toxin from Bacillus anthracis, is known as a dominant component in subunit vaccines in protection against anthrax. In order to avoid the side effects of live attenuated and killed organisms, the use of linear neutralizing epitopes of PA is recommended in order to design recombinant vaccines. The present study is aimed at determining the dominant epi...

Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed. Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...

Puziss, Milton (Fort Detrick, Frederick, Md.) and Mary B. Howard. Studies on immunity in anthrax. XI. Control of cellular permeability by bicarbonate ion in relation to protective antigen elaboration. J. Bacteriol. 85:237-243. 1963.-No elaboration of Bacillus anthracis protective antigen was demonstrated after addition of bicarbonate to cultures incubated 42 hr in a bicarbonate-free medium. Ant...

This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of trivalent vaccine using carbomer as adjuvant G. pigs an alternative cheapest animal model for quality control testing prepared vaccines formulae. Guinea were chosen experimental models develop concepts and techniques study PD50 because similarities clinical symptoms in these a...