Electron microscopic observation of thin sections of rice (Oryza sativa L.) endosperm revealed two types of protein bodies (PBs): spherical and irregular-shaped ones. Immunocytochemical localization studies using antibodies raised against purified glutelins, prolamines, and globulins indicated that the prolamines were localized in the spherical PB, whereas the irregular-shaped PB contained glut...

By screening two rice (Oryza sativa L.) seed cDNA libraries, recombinant cDNA clones encoding the rice prolamine seed storage protein were isolated. Based on cross-hybridization and restriction enzyme map analyses, these clones can be divided into two homology classes. All clones contain a single open reading frame encoding a putative rice prolamine precursor (molecular weight = 17,200) possess...

Celiac Disease is a chronic entheropathy produced by gluten intolerance, more precisely to certain proteins called prolamines, which causes atrophy of intestinal villi, malabsorption and clinical symptoms that can appear in both childhood and adulthood. This pathogeny, results from the interaction of genetic, immunologic and environmental factors, which produce the lesions on the mucosa of the ...

The use of solvents, other than water, that are capable of dissolving proteins without entering into chemical combination with them, in contrast to the usual mode of procedure, by the addition of small amounts of acid or alkali, makes it possible to investigate the properties of proteins in one of their most important states, the isoelectric state. In the present investigation, one such propert...

Of the total protein in the protein bodies of castor bean (Ricinus communis L.), approximately 40% is represented by a group of closely related albumins localized in the matrix of the organelle. This group of albumins has a sedimentation value of 2S and is resolved into several proteins of molecular weight around 12,000 daltons by sodium dodecyl sulfate-acrylamide gel electrophoresis. It has a ...

T he proximate composition and proteins of three American grain sorghum hybrids were previously described (Jones and Beckwith, 1970). We noted that the prolamine class of protein from these hybrids readily formed gels in alcohol-water systems, as well as in such solvents as dimethyl sulfoxide and 8 M urea solutions. In the alcohol-water system the addition of 1.5 M guanidine hydrochloride (GHCI...