background: rabies virus (rabv) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. l protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication.objectives: a truncated l protein of rabies virus is being cloned, expressed and purified to produce relevant ...

streptococcus iniae is a major cause of serious bacterial infections in both fish and human beings. capsular polysaccharide (cps) of s. iniae is vital to evade phagocytic clearance of the host and serves as an important protective antigen of s. iniae infection in aquatic animals. the cpsdgene was determined to be highly conservative in capsule polysaccharide operon. prokaryotic expression of th...

background: filamentous hemagglutinin (fha) is one of the most important immunoprotective antigens of bordetella pertussis (b.pertussis) and a major component of the acellular pertussis vaccine. in the present study, three overlapping recombinant fragments from the immunodominant region of fha were produced and their immunogenicity was investigated. methods: three overlapping coding sequences o...

plant lipid transfer proteins (ltps) are members of the pathogenesis-related proteins (pr-14) and some of them exhibit activity against phytopathogenic fungi. to investigate whether rice ltp2 plays a role in antifungal activity, the coding region of an iranian rice ltp2 gene was cloned into expression vector pet24-d(+) and then expressed in escherichia coli rosetta strain (de3). the potential a...

introduction: neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in human. pora is a major component of the outer membrane of n. meningitidis and functions as a cationic porin. this study aimed to clone and determine the expression of pora as the first step for producing a proper antigen in a vaccine study against n. meningitidis. methods: an approximately ...

objective: bone morphogenetic protein-7 (bmp-7) is a multifunctional growth factor predominantly recognized for its osteoinductive properties. due to the high cost of this protein, the availability of bmp-7 for treatment is limited. the heterologous production of recombinant hbmp-7 has been performed in a number of expression systems. in this study a novel form of bmp-7 was expressed in eukaryo...

Background and Aims: Influenza A virus of Orthomyxoviridae family is able to create pandemic influenza. Vaccination is the most effective way to prevent influenza virus infection. Matrix protein 2 (M2) is a homotetramer ion channel with 97 amino acids length and highly conserved among influenza viruses and is considered for development of a universal influenza vaccine. Materials and Methods: We...

turnip curly top virus (tctv) is a member of the newly established genus turncurtovirus, family geminiviridae. development of an efficient detection system is necessary to study the biological characteristics of viruses. due to the superior efficacy of the serological methods for detection of plant viruses, in this research, with the aim to facilitate the process of immunological detection of t...