نتایج جستجو برای: primer combinations
تعداد نتایج: 109699 فیلتر نتایج به سال:
abstract as hybrid spawn is propagated asexually in edible mushrooms, producers may propagate it without the permission of the owner. for the protection of the owner right, there is a great need for a molecular certificate for each commercial line. the high-resolution genotyping method of amplified fragment length polymorphism (aflp) analysis was used to prepare molecular certifications and fin...
Fungal diseases, especially leaf and stripe rusts are wheat yield reducing factors in Iran and the world. In this study, genetic diversity of 20 wheat varieties with different response to leaf and stripe rusts were studied using primers designed based on the conserved regions of plant disease resistance genes. The banding patterns of polymorphic markers were scored as dominant and number of amp...
Genetic diversity of 45 wheat varietions were studied using AFLP markers. From 11 primer combinations of PstI and Mse1for obtaining AFLP polymorphism, six primer combinations (P-CGA/M-CTA, P-ATG/M-CTG, P-ATG/M-CAT, P-AGT/M-CTA, P-AGT/M-CAG and P-ATG/M-CAG) produced polymorphic bands. Totally, 328 bands be produced that 207 bands (63.10%) were polymorphic, which showing high polymorphism among g...
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. ...
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identif...
Little information is available regarding genetic variation in wild wheat relatives from Iran. In this study, genetic diversity of 36 populations of wild einkorn wheat, Triticum boeoticum, was studied using amplified fragment length polymorphism (AFLP) primer combinations. Seventeen AFLP primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 (44%) ...
The level of polymorphism among twelve selected progenies of Gymnema sylvestre was investigated through AFLP markers by multiplexing PCR reactions using 64 (8x8) primer combinations. Fourteen primer combinations were selected as the most suitable combination for G. sylvestre. Analysis of the 12 progenies with these 14 primer pairs produced 1689 fragments of which 972 (57.5%) were polymorphic an...
Amplified fragment length polymorphism (AFLP) markers with a total of 256 EcoRI + ANN - MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 fragments per primer combination were amplified, ranging from a maximum of 226 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT...
little information is available regarding genetic variation in wild wheat relatives from iran. in this study, genetic diversity of 36 populations of wild einkorn wheat, triticum boeoticum, was studied using amplified fragment length polymorphism (aflp) primer combinations. seventeen aflp primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 (44%) ...
TG572 RFLP Probe: Five primers were designed around the RFLP probe on chromosome 7: P572F1, P572F2, P572F3, P572R1, and P572R2 (Table 1). All possible primer combinations produced a strong band with Heinz 1706 DNA between 600 bp and 1000 bp (Fig. 2). All of the primer combinations produced single bands with the exception of P572F2/P572R1, which produced two bands one at 400 bp and one at 900 bp...
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