background and objective: mmp enzymes are a family of membrane proteins that are capable of digesting extracellular matrix compounds (ecm) and basement membrane. matrilysin enzyme is the smallest member of mmp family that is encoded by mmp-7 gene (matrilysin). according to the reports, g allele of -181 a/g single nucleotide polymorphism of mmp-7 gene causes an increase in the expression of this...

Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...

Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pa...

New, quick, and inexpensive methods for genotyping novel caprine Fec gene polymorphisms through tetra-primer ARMS PCR were developed in the present investigation. Single nucleotide polymorphism (SNP) genotyping needs to be attempted to establish association between the identified mutations and traits of economic importance. In the current study, we have successfully genotyped three new SNPs ide...

Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduce...

BACKGROUND The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is a fast and economical means of assaying SNP's, requiring only PCR amplification and subsequent electrophoresis for the determination of genotypes. To improve the throughput and efficiency of T-ARMS-PCR, we combined T-ARMS-PCR with a chimeric primer-based temperature switch PCR (TSP) strategy, and used capil...

Mục tiêu nghiên cứu: Xây dựng quy trình phát hiện và bước đầu khảo sát điểm đa hình đơn nucleotide (SNP) rs7228049 trên gen SOCS6 người Việt Nam bằng phương pháp Tetra-primer ARMS PCR (T-ARMS PCR). Đối tượng Điểm gene được T-ARMS PCR, có đối chiếu với kết quả giải tự Sanger 120 khỏe mạnh. Kết quả: Đã các kiểu đồng hợp tử AA, GG dị GA tại SNP kỹ thuật PCR. Trên nhóm mạnh, tần suất alen G A lần l...