abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

s100ß a neurotrophic factor mainly released by astrocytes, has been implicated in the pathogenesis of bipolar disorder. thus, lithium may exert its neuroprotective effects to some extent through s100ß. furthermore, the possible effects of lithium on astrocytes as well as on interactions between neurons and astrocytes as a part of its mechanisms of actions are unknown. this study was undertaken...

background: in vitro model studies are becoming increasingly popular for experimental research designs. they include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. it enables  the scrutinization of intracellular signaling pathways responsible for cell death. objectives: in the ...

introduction: antibiotic supplements are regularly used in neuronal culture media to control contamination however, they can interfere with the neuronal excitability and affect electrophysiological properties. therefore, in this study, the effect of penicillin/streptomycin supplements on the spontaneous electrophysiological activity of hippocampal pyramidal neurons was examined. methods: electr...

background: current anti-cancer drug therapy results in systemic side effects due to non-specific uptake by normal healthy noncancerous tissues. to alleviate this difficulty, many attempts have been devoted to the development of new delivery systems such as polymeric nanoparticles (nps). in this study, we prepared icd-85 nps based on sodium alginate and analyzed the cytotoxic activity of icd-85...

introduction: this study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human oviduct during long-term co-culture on two-cell mouse embryos. materials and methods: using a mechanical procedure, epithelial cells from ampullary (a) and isthmic (i) regions of the human oviduct were isolated and cultured in ham's f-10 with 10% fetal calf serum (fsc). t...

cancer cell-stromal cell interaction plays a crucial role in the growth and invasion of tumor cells of various organs. it has been recently suggested that adipocytes and preadipocytes, two specific types of stromal cells, affect the biological behavior of variety of breast cancer cell lines. in spite of few investigations, there is a controversy in the literatures about the effects of adipocyte...

introduction: the purpose of this study was to cultivate bone marrow cells from two different mouse strains (nmri and balb/c) and to examine them in terms of expression of ten surface antigens from primary culture to passage 3. material and methods: 6-8 weeks old either nmri or balb/c mice were sacrificed and their bone marrows were harvested and cultivated .two weeks after culture initiation, ...

Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minimal attachment (less than 7%) occurred in serumless medium. Serum concentrations above 10% and attachment durations greater that 2 h resulted i...

Background: In vitro model studies are becoming increasingly popular for experimental research designs. They include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. It enables  the scrutinization of intracellular signaling pathways responsible for cell death. OBJECTIVES: In the ...