clinical studies have shown that in pathological conditions such as endometriosis and reproductive tract infection (male and female) there is an activation status of macrophages that produce large quantities of nitric oxide (no) in addition to other effector molecules. large amounts of no may have embryotoxic roles and produce infertility. this study was designed to evaluate the effect of diffe...

objective(s): progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. it is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. material and methods: adult female nmri mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimu...

Pre-implantation genetic diagnosis (PGD) was first pioneered at Hammersmith Hospital, London, United Kingdom, in 1989 and allows the genetic sampling and testing of the early human embryo prior to being transferred to the uterus to start a pregnancy. Although occasionally controversial, PGD is now well established in several countries and in vitro fertilisation (IVF) centres around the world. I...

Genetic disorders are a major cause of miscarriage and fetal death. Pre-implantation genetic diagnosis (PGD) can be used to diagnose genetic defects before pregnancy has occurred by creating embryos by IVF, then removing single cells which are genetically analysed using FISH or PCR. Although successful, the techniques have many difficulties because they are highly specialised and at the extreme...

Pre-implantation genetic diagnosis (PGD) is the examination in vitro of an embryo (or an egg cell prior to fertilisation) in order to exclude a genetic condition in case a very high risk of that condition is known. PGD can only be used in combination with in vitro fertilisation (IVF). If a genetic abnormality in the form of a monogenetic disease such as Huntington’s disease or cystic fibrosis o...

a bstract background: non‑medical utilization of pre‑implantation genetic diagnosis (pgd), like sex selection, is increasing, therefore it is necessary to follow‑up the health and outcome of fertilization and newborn’s birth followed pgd. the aim of this study was to evaluate the outcome of fertilization after sex selection by pgd and the relation between the age of parents and the outcome of f...

Background: Regulation of gene expression in mammalian embryos is not completely known. Pre-implantation embryos need maternal RNA and proteins synthesized during oogenesis, to regulate development before mater-embryo transition, as the grown oocyte and the 1-cell zygote are transcriptionally silent. There are some oocyte-specific genes called maternal effect genes which may account for this ea...

Objective(s): Progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. It is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. Material and Methods: Adult female NMRI mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimu...

Objective(s): To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4). Materials and Methods: Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10-9 M melatonin) and Luzindole-treated (10-9 M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the leve...