A full-length cDNA copy of the genome of Potato leafroll virus (PLRV) was introduced into the genome of tobacco and potato plants by Agrobacterium tumefaciens-mediated transformation. Transgenic lines were obtained in which the transgene was readily detected by PCR with DNA extracted from T(1) tobacco seedlings and clonally multiplied potato plants. PLRV-specific genomic and sub- genomic RNAs, ...

Evaluation of Some Characteristics Substantial Equivalence a Salinity-Resistant Transgenic Potato

Transgenic plants expressing a foreign gene are a suitable system for the production of relevant immunogens in high amounts that can be used for the development of a new generation of vaccines against a variety of infectious diseases. In the present study, the expression of the nucleocapsid (N) protein of hantavirus serotype Puumala in tobacco and potato plants was investigated. Transgenic toba...

the performance and flight behavior of the potato aphid macrosiphum euphorbiae was studied on the 'superior-bt' line transgenic for the cryiiia toxin of bacillus thuringien-sis (bt), resistant to the colorado potato beetle; and non transformed 'superior' line which served as control. mortality of the treated aphids was negligible and potato lines did not affect the development time of m. euphor...

Sweet potato belongs to Ipomoea series batatas and is thought to have originated in Central America and Northern South America. World sweet potato production is around 106 million tons in an area of about 8.1 million ha. Asia is the world’s largest sweet potato-producing region and China is the world’s leading sweet potato-producing country accounting for about 76% of production. Sweet potato h...

Survival and fecundity of Colorado potato beetle adults, Leptinotarsa decemlineata (Say), that had or had not fed previously on non-transgenic potato before exposure to transgenic potato containing the Bacillus thuringiensis subsp. tenebrionis Cry3A toxin (Bt) was investigated. In the laboratory, < 5% of first-generation adults survived after two weeks when restricted to Bt foliage since eclosi...

A chimeric gene containing the patatin promoter and the transitpeptide region of the small-subunit carboxylase gene was utilized to direct expression of Escherichia coli glycogen synthase (glgA) to potato (Solanum tuberosum) tuber amyloplasts. Expression of the glgA gene produd in tuber amyloplasts was between 0.007 and 0.028% of total protein in independent potato lines as determined by immuno...

Abiotic stresses have negative effects on potato growth and production. To enhance the abiotic stress tolerance of the commercial potato cultivar, Desiree, rd29A::AtDREB1A transgenic lines have been developed. The salinity and freeze tolerance of these transgenic lines has previously been demonstrated; however, their dehydration tolerance remains to be elucidated. First of all, we have tackled ...