background and aims: this research aimed to study a possible link between endometriosis and polymorphism of the progesterone receptor gene. materials and methods: the control group consisted of 86 women without endometriosis and the case group comprised 86 patients with a diagnosis of endometriosis by laparoscopy. genotypes for alu insertion polymorphisms (a1/a1, a1/a2 and a2/a2) were described...

conclusions obtained data demonstrated that the designed assay is an accurate, easy, rapid, and cost-effective tool for detecting v. cholerae that can serve as an alternate to current bacteriological tests. background several epidemic and endemic cases have been reported involving vibrio cholerae (v. cholerae) as a causative organism in serious diarrheal diseases with high mortality. hence, qui...

Polymerase gamma is a mitochondrial DNA polymerase, that responsible for the replication of (mtDNA). It encoded by POLG gene, on chromosome 15q25. Various mutations in this gene have been described, with varied phenotypic manifestations. The triad sensory ataxic neuropathy, dysarthria, and ophthalmoparesis (SANDO) has reported only small group patients mutations. We report case male, who presen...

although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this stu...

a new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising dna annealing-ligation and augmentation by polymerase chain reaction was introduced. the sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. first, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

background: recently, the use of t7 rna polymerase instead of other viral and cellular promoters is increasing due to high efficacy of transcription in the cell cytoplasm by this polymerase. in order to translate the transcripts produced by t7 rna polymerase in mammalian cell lines, it is necessary to include internal ribosome entry site (ires) sequences. in addition, if sequence of poly a sign...

conclusions this study showed that the presence of mutation at codons rtl80v, rtl180m and rtm204v in a, b and c domains is associated with higher viral load and resistance to lamivudine (3tc) respectively. results about 6% (3 of 50) of samples were hbeag positive and 94% (47 of 50) of patients were hbeag negative by elisa method. the patients' alt level was between 16 and 95 iu/l with the mean ...

five common tetracycline resistance genes tet(a), tet(b), tet(m), tet(o) and tet(s) were studied by polymerase chain reaction in 100 bacteria isolated from iranian fish farms. in the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. nine isolates out of 46 aeromonas spp. contained either tet(a/m/s) resistant genes as follows: tet(a) in a. ...

by application of modern recombinant dna technology, especially the polymerase chain reaction (pcr)/dot-blot hybridization techniques, we have investigated the molecular basis of β-thalassemia from four different regions of iran: central, south-east, south and north. in this study, the dna samples were isolated from patients and for the identification of the mutations, the 6 oligonucleotide pro...