results our study showed that the sensitivity and specificity of taqman real time pcr was 96% and 95% respectively. these values were 89% and 90, 38% and 100%, 6% and 100% for pcr, culture and microscopy, respectively. conclusions our study showed that sensitivity of taqman real time pcr was higher that pcr, culture and microscopy but specificity of culture and microscopy was more than pcr and ...

dear editor-in-chief we read with interest the study by khazaei et al. (1) in which the authors have nicely concluded that pcr is more sensitive test than ziehl-neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (tb). they have rightly pointed to use pcr, selectively, in acidfast bacilli negative paucibacillary forms of tb. however, we intend to highlight few ...

background: taq dna polymerase is a very important enzyme for molecular biological studies such as dna amplification and dna sequencing by the pcr. it is a standard enzyme that is used in 90% of molecular biology labs today. the aim of this study was to produce taq dna polymerase enzyme in e. coli by a reliable, practical, simple and low cost method.materials and methods: in this study, the taq...