Proteins and peptides are useful research and therapeutic tools, however applications are limited because delivery to the desired location is not easily achievable. There are two hurdles in protein/peptide delivery to the brain: the blood-brain barrier and intracellular penetration. Penetration to both brain and the intracellular space can be achieved by adjusting hydrophilicity, and small mole...

Polyethylenimines are cationic polymers with potential as delivery vectors in gene therapy and with proven antimicrobial activity. However, the antiviral activity of polyethylenimines has not been addressed in detail thus far. We have studied the inhibitory effects of a linear 25-kDa polyethylenimine on infections with human papillomaviruses and human cytomegaloviruses. Preincubation of cells w...

A number of agents are now available for use in protecting against ionizing radiation. These radiation-protective agents, however, have many adverse effects. Efforts have been made to develop new radiation-protective agents for medical application. Here, we investigated whether a compound, polyethylenimine (PEI), which activates Toll-like receptor 5 (TLR5)-mediated NF-kB signaling pathways, cou...

objective polyethylenimine (pei), a readily available synthetic polycation which has high transfection efficiency owing to its buffering capacity was introduced for transfection a few years ago. but it has been reported that pei is cytotoxic in many cell lines. in this study, in order to enhance the transfection efficiency of 10 kda pei and reduce its toxicity, hydrophobic residues were grafted...

A polyethylenimine adjuvant modified by mannose, which may be used in the study of vaccine adjuvant, was prepared this paper. Its structure characterized infrared spectroscopy and its micromorphology observed transmission electron microscopy. series mannose-modified polyvinylimine were according to molar ratio primary amine groups between mannose polyvinylimine, their storage stability at 4°C. ...

In this work the stability of TiN and TiC nanopowders in isopropyl alcohol as well as the fabrication of dense and well adhered thin coatings based on TiN and TiC by electrophoretic deposition (EPD) have been evaluated in terms of zeta potential and mass deposited when hydrazine is added. The surface of TiN and TiC nanoparticles has been modified to improve the dispersion in isopropyl alcohol a...

Scanning force microscopy was used to examine DNA condensates prepared with varying stoichiometries of lipospermine or polyethylenimine in physiological solution. For the first time, individual DNA strands were clearly visualized in incomplete condensates without drying. Using lipospermine at sub-saturating concentrations, discrete nuclei of condensation were observed often surrounded by folded...

The application of silicalite for improvement of working characteristics of conductometric enzyme biosensors for determination of sucrose was studied in this research. Biosensors based on different types of silicalite-modified electrodes were studied and compared according to their analytical characteristics. Polyethylenimine/glutaraldehyde/silicalite-modified biosensors showed higher sensitivi...

Low-molecular-weight polyethylenimine has lower cytotoxicity than high molecular weight polyethylenimine, but it is not an efficient transfection agent because of limitations of DNA delivery into the cytoplasm. Therefore, in the present study, the hydrophobic modification of low-molecular-weight polyethylenimine (PEI 2 kDa [PEI2]) by cholic acid (ChA) was performed to form PEI2-ChA, and in vitr...

chitosan-graft-polyethylenimine (chi-g-pei) copolymer has been used for theimprovement of low transfection efficiency of chitosan. the present study aims to test thepulmonary toxicity and efficiency of chi-g-pei as an aerosol gene carrier. mice were exposedto aerosol containing green-fluorescent protein (gfp)-polyethylenimine (pei) or gfp-chig-pei complexes for 30 min during the development of ...