Live Trypanosoma cruzi amastigotes hydrolyzed p-nitrophenylphosphate (PNPP), phospho-amino-acids and 32P-casein under physiologically appropriate conditions. PNPP was hydrolysed at a rate of 80 nmol.mg-1.h-1 in the presence of 5 mM MgCl2, pH 7.2 at 30 degrees C. In the absence of Mg2+ the activity was reduced 40% and we call this basal activity. At saturating concentration of PNPP, half-maximal...

The synthetic peptide PnPP-19 comprehends 19 amino acid residues and it represents part of the primary structure of the toxin δ-CNTX-Pn1c (PnTx2-6), isolated from the venom of the spider Phoneutria nigriventer. Behavioural tests suggest that PnPP-19 induces antinociception by activation of CB1, μ and δ opioid receptors. Since the peripheral and central antinociception induced by PnPP-19 involve...

Rumah sebagai salah satu kebutuhan dasar manusia, idealnya harus dimiliki oleh setiap keluarga, terutama bagi masyarakat yang berpenghasilan rendah dan tinggal di daerah padat penduduk perkotaan. Negara juga bertanggung jawab dalam menyediakan memberikan kemudahan perolehan rumah melalui penyelenggaraan perumahan kawasan permukiman termasuk Pegawai Negeri Pada Polri (PNPP). Penelitian telah dil...

PURPOSE To develop a bio-assay that would be able to directly test gastrointestinal and/or dissolution samples to determine lipase activity and inhibition by Orlistat. METHODS Enzyme assays were performed with porcine pancreatic lipase and para-Nitrophenyl Palmitate (pNPP) in pH 8.0 reaction buffer at 37°C. Substrate hydrolysis was monitored by absorbance changes at 410 nm. The dissolution of...

Using para-nitrophenyl phosphate (pNPP) as a substrate for enzymatic activity, we sought to identify CaN in Paramecium. We isolated three different pNPP-phosphatases from the soluble fraction of Paramecium cells by anion-exchange and affinity column chromatographies. One, pNPP-phosphatase Peak I, is very similar to mammalian CaN. Divalent cation dependency, inhibition by calmodulin (CaM) antago...

Phosphoester hydrolysis is an important chemical step in DNA repair. One archetypal molecular model of phosphoesters para-nitrophenylphosphate (pNPP). It has been shown previously that the presence metal oxide [Mo7O24]6− may catalyse pNPP through partial decomposition polyoxomolybdate framework resulting a [(PO4)2Mo5O15]6− product. Real-time monitoring catalytic system using electrospray ionisa...

Real time monitoring of electrolyte resistance changes during hydrolysis of 4-nitrophenylphosphate (pNPP) by alkaline phosphatase (ALP) bound on paramagnetic-beads was performed into a small dielectric channel. The reaction kinetic fit with a non-competitive substrate-inhibition equation. Michaelis-Menten apparent constant, KM(app), was determined as 0.33±0.06mM and the maximum apparent rate, V...

Total flower and pod numbers (TFPN) effective per plant (PNPP) are among the most important agronomic traits for soybean production. However, underlying genetic mechanism remains unclear. In this study, we constructed a recombinant inbred line population derived from cross between JY73 (high TFPN) TJSLH (low to map loci two traits. total, six QTL TFPN five PNPP were identified, which on chromos...

The skeletal isoenzyme of human alkaline phosphatase was partially purified from the serum of a patient with Paget’s disease. The pH dependence of the reaction with p-nitrophenyl phosphate (PNPP) suggests that either the reaction mechanism involves 2 dissociable residues on the enzyme (8.75 > pK1 > 8.2; 9.35 > pKz > 8.75), or that the monoanion is the active form of the phosphoryl substrate. In...

The protein Ser/Thr phosphatase family contains three enzymes called PP2A, PP4, and PP6 with separate biological functions inferred from genetics of the yeast homologues Pph21/22, Pph3, and Sit4. These catalytic subunits associate with a common subunit called alpha4 (related to yeast Tap42). Here, we characterized recombinant PP6 and PP2A catalytic monomers and alpha4.phosphatase heterodimers. ...