A simulation approach is introduced for directly determining reaction paths and stationary points on potential of mean force (PMF) surfaces associated with molecular events that occur in complex environments. The nudged elastic band approach was employed to search for steepest descent paths on the PMF surface using the relevant PMF derivatives from a series of local simulations. The steepest de...

This note introduces the use of distributed polarization x-talk measurement for raw polarization maintaining fiber (PMF) quality inspection, PMF coil winding process monitoring, PMF coil adhesive curing and stress release monitoring, overall PMF coil quality inspection, and temperature induced stress determination. PMF birefringence temperature variation can also be precisely measured. The use ...

The proton motive force (PMF) renders protein translocation across the Escherichia coli membrane highly efficient, although the underlying mechanism has not been clarified. The membrane insertion and deinsertion of SecA coupled to ATP binding and hydrolysis, respectively, are thought to drive the translocation. We report here that PMF significantly decreases the level of membrane-inserted SecA....

Previous studies confirm that pulsed magnetic field (PMF) accelerates functional recovery after a nerve crush lesion. The contention that PMF enhances the regeneration is still controversial, however. The influence of a new PMF application protocol (trained PMF) on nerve regeneration was studied in a model of crush injury of the sciatic nerve of rats. To determine if exposure to PMF influences ...

We constrain a primordial magnetic field (PMF) generated during a phase transitions (PT) using big bang nucleosynthesis (BBN) bounds on the relativistic energy density. The amplitude of the PMF at large scales is determined by the spectral shape of the PMF spectrum outside its maximal correlation length scale. Even if the amplitude of the PMF at 1 Mpc is small, PT generated PMFs can leave obser...

Myeloproliferative neoplasms (MPN) are chronic myeloid cancers thought to arise at the level of CD34+ hematopoietic stem/progenitor cells. They include essential thrombocythemia (ET), polycythemia vera (PV) and primary myelofibrosis (PMF). All can progress to acute leukemia, but PMF carries the worst prognosis. Increasing evidences indicate that deregulation of microRNAs (miRNAs) might plays an...

BACKGROUND Limited data exist regarding impact of IDH mutations in Philadelphia-negative myeloproliferative neoplasms (Ph-negative MPNs). Prognostic significance of IDH mutations was asessed in 184 Ph-negative MPN patients - 107 essential thrombocythemia (ET) and 77 primary myelofibrosis (PMF). METHODS High-resolution melting (HRM) analysis was used to detect IDH1 and IDH2 mutations. RESULT...

Identification of proteins by mass spectrometry (MS) is an essential step in proteomic studies and is typically accomplished by either peptide mass fingerprinting (PMF) or amino acid sequencing of the peptide. Although sequence information from MS/MS analysis can be used to validate PMF-based protein identification, it may not be practical when analyzing a large number of proteins and when high...

PURPOSE To investigate whether low frequency pulsed magnetic field (PMF) exposures produce alterations in the cell killing induced by ultraviolet C (UVC) radiation. MATERIALS AND METHODS MCF-7 breast cancer cells of exponentially growing cultures were exposed to PMF (25 Hz, 0.75 mT) and UVC (from 6.6 J/m2 to 59.4 J/m2) in two different protocols: (a) cells were exposed to PMF for 30 min and t...