نتایج جستجو برای: plasmid transformation
تعداد نتایج: 253370 فیلتر نتایج به سال:
introduction: resistance of pathogenic organisms to countenance antibiotics has become a worldwide problem with serious consequences on the treatment of infectious diseases. the aim of this study was to evaluate antibiotic resistance and also the detection of transferred antibiotic resistance by plasmid in clinical proteus isolates. materials and methods: a total of 250 urine samples were colle...
Background and purpose: Sea anemones of the genus Actinia are commonly found in the intertidal zone of the northern rocky coast of Spain. Actinia fragacea is one of the most important species that secretes a toxic protein, fragaceatoxin C (FraC), for defense and hunting purposes. The aim of this study was transformation of plasmid containing the gene encoding the FraC to Escherichia coli bacter...
Background & Objective: Transformation of plasmid DNA into bacterial competent cells is a key technique for molecular cloning. Transformation can be achieved using either chemical or physical methods, e.g., electroporation. The rate of success in these methods depends on experience and attention to method’s details. Therefore, the higher the efficiency and quality of a transformation method, ...
Spontaneous plasmid transformation of Escherichia coli is independent of the DNA uptake machinery for single-stranded DNA (ssDNA) entry. The one-hit kinetic pattern of plasmid transformation indicates that double-stranded DNA (dsDNA) enters E. coli cells on agar plates. However, DNA uptake and transformation regulation remain unclear in this new type of plasmid transformation. In this study, we...
Background: Interferon beta is one of the most important members of group I interferons and is the main drug for multiple sclerosis treatment. Interferon beta has short half life and this compels patients to make frequent use of medicine. According to its clinical usage there is broad effort to improve translation level and protein production. There are several important factors which effect pr...
Background & Aim: Toxoplasmosis is caused by a protozoan parasite called toxoplasma gondii. SAG1 is an antigen which only exists in tachyzoite stage. This antigen which has two glycoforms is a conformational antigen. The aim of this research is to study cloning of SAG1 gene in PTZ57R and transformation of recombinant plasmid in E.coli(TG1 & DH5a). Material & Method: Tachyzoites of T.gondii ...
Escherichia coli as a plasmid recipient cell was dispersed in a chrysotile colloidal solution, containing chrysotile adsorbed to plasmid DNA (chrysotile-plasmid cell mixture). Following this, the chrysotile-plasmid cell mixture was dropped onto the surface of an elastic body, such as agarose, and treated physically by sliding a polystyrene streak bar over the elastic body to create friction. Pl...
Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with un...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید