Growth characteristics, oxygen exchange, and carbohydrate and chlorophyll contents were determined 30 days after subculturing of single node-derived plantlets of Solanum tuberosum cv Haig cultivated in vitro. Cultivation conditions were: (a) photomixotrophy in closed vessel, (b) photomixotrophy in closed vessel on medium supplemented with silver thiosulfate, (c) photomixotrophy in aerated vesse...

An efficient in vitro regeneration protocol was established for Swertia chirayita. The present study describes the regeneration of S. chirayita from shoot tip explants collected from wild grown plantlets. The highest number of shoots (26.10 ± 0.73) were obtained on Murashige and Skoog basal medium supplemented with 6-benzyladenine (BA) (0.5mg/l) in combination with gibberellic acid (GA3) (1.0 m...

Physiological characteristics of in vitro and field cultivated Leucojum aestivum l. Plants stanilova m. summary. A living collection of Leucojum aestivum L. (Amaryllidaceae) was established at the Institute of Botany, Sofia, in 2001. In vitro cultures originating from a single mother bulb have been maintained since 2003 on MS-based agar-solidified medium supplemented with 30 g L-1 sucrose, 2 mg...

Aloe plants can be grown autotrophically in a maintenance-free, sterile, ex vitro environment. Excel, 15-5-15 plus minors fertilizer formulation and Plant Preservative Medium (PPM) biocide was used as a very economical basal medium. In vitro micropropagated plantlets were regenerated in Excel media with 2 g /L sucrose and 2.2 μM 6-Benzylamino purine (BA) and used to initiate ex vitro cultures i...

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 we...

The aim of our study was to consider the efficiency of randomly amplified polymorphic DNA (RAPD) analysis to show the differences between Rhododendron mutants and control plants. The mutant plantlets were selected due to their better In vitro shoot and root regeneration relative to control in both varieties. Six mutant plantlets from Alfred and seven mutant plantlets from Paars variety were sel...

plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. photoautotrophic and photomixotrophic systems could diminish these problems. the purpose of this study was to increase the chlorophyll...