نتایج جستجو برای: plant protease
تعداد نتایج: 438745 فیلتر نتایج به سال:
the date palm fruit stalk borer is one of the most important pests of date palm in the world. biochemical properties of digestive proteases in oryctes elegans prell larvae were investigated in this research and optimal total proteolytic and trypsin activities were obtained at ph 9.0 and 11.0, respectively. activity staining of protease on sds-page showed one isoform. also, zymogram pattern of t...
proteases catalyze the hydrolysis of peptide bonds in proteins. they are widely distributed in nature, nearly in all plants, animals and microorganisms. plant latex is a rich source of proteases. latex production is unique property of euphorbian plant, which could be a potential source of proteolytic enzymes. the highest proteolytic activity was observed in the latex of pedilanthus tithymaloide...
Isolating and cloning of plant protease inhibitor (PIs) genes and transforming them to the genome of other plants have paved the way for producing resistant transgenic plants against pests. Knowing that cystatins act as inhibitor factor against cysteine protease, short and long cystatin genes were isolated from maize mRNA. By using specific primers, cDNA of these genes were constructed and clon...
No evidence has been found to show that the protease is a mixture ofenzymes or that the protease of one plant is distinguishable from another. It has been customary to name a protease after the generic name of the plant in which it is found. This custom, both here and in future work, implies an indefinite extension in the number of proteases. It is therefore recommended that the proteases descr...
Isolating and cloning of plant protease inhibitor (PIs) genes and transforming them to the genome of other plants have paved the way for producing resistant transgenic plants against pests. Knowing that cystatins act as inhibitor factor against cysteine protease, short and long cystatin genes were isolated from maize mRNA. By using specific primers, cDNA of these genes were constructed and cl...
Natural senescence is associated with several fold increase in activity of protease and is characterized by the degradation and loss of proteins therefore plant proteases have been a common studied interestingly. Protease from senesced leaves of the papaya plant (Carica Papaya) was partially purified by ammonium sulfate precipitation. It showed 15 fold purification. Optimum protease activity wa...
The plant apoplast forms a protease-rich environment in which proteases are integral components of the plant defense response. Plant pathogenic oomycetes, such as the potato (Solanum tuberosum) and tomato (Lycopersicon esculentum) pathogen Phytophthora infestans, secrete a diverse family of serine protease inhibitors of the Kazal family. Among these, the two-domain EPI1 protein was shown to inh...
Medicinal plants especially leaves are used in traditional medicine for rapid anti-viral therapy against infectious diseases. Protease, a potential candidate in medicinal plants is not so for studied in leaves. So an attempt was made to determine the protease activity of various medicinal plants especially leaves. Buffers of different pH range were used for extraction of the leaves to identify ...
Plants are increasingly used as alternative expression hosts for the production of recombinant proteins offering many advantages including higher biomass and the ability to perform post-translational modifications on complex proteins. Key challenges for optimized accumulation of recombinant proteins in a plant system still remain, including endogenous plant proteolytic activity, which may sever...
Precursor protease vesicles are plant-specific compartments containing precursors of enzymes that are thought to participate in the degradation of cellular components in organs undergoing senescence. We report in vivo evidence that the precursor protease vesicle-localized vacuolar processing enzyme-gamma (VPEgamma) is critical for maturation of the plant vacuolar protease AtCPY. We also provide...
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