background: this study was designed to investigate the efficacy of pentoxifylline (ptx) in the treatment of irradiated wounds. materials and methods: ten female pigs of the large white strain were used in these investigations. six skin fields, 4 * 4 cm and separated from each other by 4 cm, were marked out on each flank and those on left flank were irradiated with 18gy single dose of 250 kv x-r...

background: biting habit of mosquitoes plays an important role in the epidemiology of mosquito-borne diseases. mosquitoes use a set of elaborate sensory modalities to find their preferred hosts by exploiting cues emanating from a nearby host. it has been suggested that the chemical profile of skin can provide further support for anthropophilic mosquito species to find their suitable hosts. this...

Animal models are important tools to predict human in vivo percutaneous absorption/penetration. Monkey, pig, rat, rabbit, guinea pig, hairless rodents, such as hairless rat, hairless mouse, hairless guinea pig and hairless dog, are among the most used animals for this purpose. Each animal model has its own advantages and weakness or limitation. To better correlate animal data with human skin ab...

introduction: it is believed that injury potential has a regulatory role in wound healing process and the application of exogenous electrical stimulation may serve to mimic the natural endogenous bioelectric current so that can improve the wound healing. up to now, this hypothesis has not been researched in surgically acute wounds. materials and methods: thirty nine male guinea pigs were random...

BACKGROUND Allergic sensitization and reactions to guinea pig (Cavia porcellus) have been well documented in laboratory animal handlers, primarily manifesting as rhinitis, conjunctivitis, and asthma. Severe allergic reactions, however, are rare. METHODS We report two patients with severe allergic reactions following non-occupational exposure to guinea pigs. The first patient, an 11-year-old f...

Collagen is an ubiquitous structural protein, which constitutes about 25-30% by weight of all proteins in the mammalian body. It functions as an important supporting element in vertebrate tissues. Using the method of cleaving the protein chains at the methionyl residues with cyanogen bromide (CNBr), many species of collagen from different sources have been isolated and characterized, e.g. rat s...

The major concanavalin A-binding component in urea/deoxycholate/mercaptoethanol extracts of pig skin was a collagenous disulphide-cross-linked glycopolypeptide with an apparent molecular mass of 150 kDa and a pI of 5.5. Antiserum against the electrophoretically purified glycopolypeptide gave strong dermal staining similar to that seen with fluorescent concanavalin A. Immunocytochemical labellin...

Split-thickness pig skin was transplanted on severe combined immunodeficient mice so that pig dermal microvessels spontaneously inosculated with mouse microvessels and functioned to perfuse the grafts. Pig endothelial cells in the healed grafts constitutively expressed class I and class II major histocompatibility complex molecules. Major histocompatibility complex molecule expression could be ...

The insertion and expression of genes in the epidermis may have a variety of therapeutic uses, including the treatment of skin diseases. Here we show that when both human skin organ cultures and human skin grafts on immunocompromised mice are injected with naked DNA, the DNA is taken-up and genes are expressed in the epidermis in a manner similar to both pig skin injected in vivo and injected p...

The simultaneous application of ultrasound and the surfactant sodium lauryl sulfate (referred to as US/SLS) to skin enhances transdermal drug delivery (TDD) in a synergistic mechanical and chemical manner. Since full-thickness skin (FTS) and split-thickness skin (STS) differ in mechanical strength, US/SLS treatment may have different effects on their transdermal transport pathways. Therefore, w...