نتایج جستجو برای: pichiapastoris

تعداد نتایج: 20  

2013
Hussam H. Shaheen Bianka Prinz Ming-Tang Chen Tej Pavoor Song Lin Nga Rewa Houston-Cummings Renee Moore Terrance A. Stadheim Dongxing Zha

State-of-the-art monoclonal antibody (mAb) discovery methods that utilize surface display techniques in prokaryotic and eukaryotic cells require multiple steps of reformatting and switching of hosts to transition from display to expression. This results in a separation between antibody affinity maturation and full-length mAb production platforms. Here, we report for the first time, a method in ...

Journal: :iranian biomedical journal 0
علی اکبر شعبانی ali akbar shaebani robert w. mcmaster دلاور شهباززاده delavar shahbazzadeh محسن کریمی mohsen karimi فریدون مهبودی fereidoun mahboudi

leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. several approaches toward vaccine development against this disease have been taken. glycoprotein (gp63) is conserved among diverse species of leishmania and has induced immunological responses in murine models. therefore, this glycoprotein has been considered as a second generat...

Journal: :iranian journal of biotechnology 2014
praveen kumar vemuri suryanarayana veeravalli

background: over the past century, the areas of genomics, proteomics and lipids have captured the attention of investigators worldwide. carbohydrates, have recently received increased attention through the expanding field of glycobiology; probably because they are very complex and not encoded in the genome. objectives: the purpose of this study was to express and purify recombinant human galect...

2014
Enedina Nogueira de Assunçao Santos Maria Cristina dos Torres Fernando Araripe Astolfi-Filho Spartaco

Background There are indications, according to WHO, that diabetes will reach 333 million people in 2025, and already is among one of the main death causes in the world. In 2030 in Latin America, diabetes mellitus will be the second cause of deaths [1]. The actions to increase the production of insulin are strategic to respond the high demands for what we have to face in the near future [2]. P. ...

2013
Song Yang Ye Kuang Hongbo Li Yuehong Liu Xiaoyan Hui Peng Li Zhiwu Jiang Yulai Zhou Yu Wang Aimin Xu Shiwu Li Pentao Liu Donghai Wu

Pichiapastoris is one of the most widely used expression systems for the production of recombinant secretory proteins. Its universal application is, however, somewhat hampered by its unpredictable yields for different heterologous proteins, which is now believed to be caused in part by their varied efficiencies to traffic through the host secretion machinery. The yeast endoprotease Kex2 removes...

2010
Byung-Kwon Choi Piotr Bobrowicz Robert C. Davidson Stephen R. Hamilton David H. Kung Huijuan Li Robert G. Miele Juergen H. Nett Stefan Wildt Tillman U. Gerngross Randy Schekman Kai J. Miller Gerwin Schalk Eberhard E. Fetz Marcel den Nijs Jeffrey G. Ojemann Rajesh P. N. Rao

APPLIED BIOLOGICAL SCIENCES PNAS is publishing an Editorial Expression of Concern regarding the following article: “Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichiapastoris,” by Byung-Kwon Choi, Piotr Bobrowicz, Robert C. Davidson, Stephen R. Hamilton, David H. Kung, Huijuan Li, Robert G. Miele, Juergen H. Nett, Stefan Wildt, and Tillman U. Gerngross...

2010
Byung-Kwon Choi Piotr Bobrowicz Robert C. Davidson Stephen R. Hamilton David H. Kung Huijuan Li Robert G. Miele Juergen H. Nett Stefan Wildt Kai J. Miller Gerwin Schalk Eberhard E. Fetz Marcel den Nijs Jeffrey G. Ojemann Rajesh P. N. Rao

APPLIED BIOLOGICAL SCIENCES PNAS is publishing an Editorial Expression of Concern regarding the following article: “Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichiapastoris,” by Byung-Kwon Choi, Piotr Bobrowicz, Robert C. Davidson, Stephen R. Hamilton, David H. Kung, Huijuan Li, Robert G. Miele, Juergen H. Nett, Stefan Wildt, and Tillman U. Gerngross...

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