AIM This study was conducted to find the best suited freely available software for modelling of proteins by taking a few sample proteins. The proteins used were small to big in size with available crystal structures for the purpose of benchmarking. Key players like Phyre2, Swiss-Model, CPHmodels-3.0, Homer, (PS)2, (PS)(2)-V(2), Modweb were used for the comparison and model generation. RESULTS...

background: asthma is the main reason of disability, health resource exploitation and low quality of life for those who are affected. it is estimated that nearly 300 million people in the world are suffering from asthma. studies have identified 18 genomic regions and more than 100 genes associated with asthma. among these candidate genes, il-17f plays a very interesting role in asthma. this stu...

The science of metabolomics is exponentially growing with the boost in knowledge base of metabolites and development of bioinformatics data analysis tools. Human ACAA2 gene codes for a 397 amino acid protein, 3Ketoacyl-CoA thiolase, which is a mitochondrial enzyme responsible for catalyzing the thiolytic cleavage of 3-ketoacyl-CoA by thiolase, the last reaction of fatty-acid β-oxidation. Bioinf...

Desulfurization protein named DszC from Rhodococcus erythropolis is the key enzyme for biodesulforization of dibenzothiophene (DBT) in 4S pathway, which is a pathway with four enzymes. DszC enzyme biodesulfurizes DBT and its derivatives in oil components and biphasic systems. It functions well at the oil- water interface. In this study point mutation performed in DszC enzyme regarding to increa...

The cancer profile in the Indian state of Uttarakhand reveals that the breast cancer is the most prevalent type of cancers in females followed by cervical and ovarian type. Literature survey shows that the E6 protein of Human Papilloma Virus-16 (HPV-16) is responsible for causing several forms of cancer in human. Therefore, it is of interest to screen HPV-16 E6 target protein with known natural...

Background: The Mycobacterium tuberculosis complex (MTBC) bacteria include the slowly growing, host-associated tuberculosis, Bovis, microti, africanum, pinnipedii. Aim: Comparative Functional Genomics Studies for understanding Hypothetical Proteins in variant microti 12. Objective: A computational genomics study was performed to understand 247 hypothetical protein genes. annotation of virtual p...

Background: Genetic analysis in mice has lead to find about maternal effect genes such as Filia. Filia knock out mice have a 50% decrease in fertility. Filia dysfunction causes disorders in pre-implantation development. Mutations in human Filia gene, cause FBHM (Familial Biparental Hydatidiform Mole) in women. Filia protein in mice is homologous to that of rat and human, so this idea has emerge...

Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate biochemical properties, critical residues, and catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence analyzed ProtParam, SignalP, Phyre2 tools signal peptide, secondary structure, respectively....