Moringa oleifera has been shown to have a variety of medicinal benefits, including hepatoprotective effects. Phenylhydrazine used cause hepatotoxicity in experimental models. In this study, an aqueous extract was evaluate the ameliorating properties phenylhydrazine-induced liver damage by assessing enzymes and histoarchitecture employing twenty-five (25) mature Wistar rats were divided into fiv...

Tyrosinase was found to catalyze the oxidation of phenylhydrazine to phenol in a reaction that did not resemble those typically performed by tyrosinase. The kinetics of this reaction was investigated by measuring the initial velocity of the formation of phenol (25 °C). The values of k cat and K M for the oxidation of phenylhydrazine were obtained as 11.0 s(-1) and 0.30 mM, respectively. The gen...

The cancer potencies of phenylhydrazine and phenylhydrazine hydrochloride were estimated from dose-response data from three data sets: lung tumors among male and female mice (combined) exposed orally to phenylhydrazine hydrochloride (Clayson et al., 1966) and blood vessel tumors among male and female mice exposed orally to phenylhydrazine hydrochloride (Toth and Shimizu, 1976). Studies of pheny...

It is well known that phenylhydrazine induces hemolytic anemia. This is thought to result from the reaction of phenylhydrazine with hemoglobin. The accompanying oxidation of phenylhydrazine leads to the formation of a number of products, including benzene, nitrogen, hydrogen peroxide, superoxide anion and the phenyl radical. The products formed depend critically on the conditions of the experim...

An amperometric horseradish peroxidase (HRP) inhibition biosensor has been substantially constructed by the help of N,N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS). The preparation steps and the biosensor response to phenylhydrazine were monitored by electrochemical impedance spectroscopy (EIS), cyclic voltammetry, and chronoamperometry. The proposed biosensor could be applied to...

Phenylhydrazine is a genotoxic impurity commonly used as a raw material in active pharmaceutical ingredients. In this study, a novel method, using inductively coupled plasma-mass spectrometry combined with two-dimensional liquid chromatography (LC-ICP-MS), was employed for the quantitation of residual phenylhydrazine in antipyrine. The compound 2,3,5-triiodobenzoyl chloride, which contains thre...

BACKGROUND Phenylhydrazine, a hemolytic agent, is widely used as a model of experimental hyperbilirubinemia. Palm tocotrienol-rich fraction (TRF) was shown to exert beneficial effects in hyperbilirubinemic rat neonates. AIM To investigate the effects of palm TRF supplementation on hepatic bilirubin-metabolizing enzymes and oxidative stress status in rats administered phenylhydrazine. METHOD...

Lipid peroxidation, hemolysis and thiol contents were studied in intact goat erythrocytes exposed to phenylhydrazine. An increase in lipid peroxidation, hemolysis and thiol content was observed after phenylhydrazine treatment of erythrocyte. Extracellular Ca potentiates the phenylhydrazine-induced lipid peroxidation and hemolysis of erythrocytes significantly. Ca does not influence much the thi...

Phenylhydrazine is oxidized stoichiometrically by two equivalents of ferricyanide to produce phenyldiimide and ferrocyanide. This reaction permits accurate determination of the absorption maxima and molar absorption coefficients of phenyldiimide in aqueous solution. These values are identical or extremely similar to those of phenyldiimide produced by decarboxylation of phenylazoformic acid. Kno...