Abstract Peroxisomes are very important organelles in eukaryotic cells and participate various biological processes, including pathogen–host interactions. A variety of proteins involved peroxisome proliferation metabolic activity within peroxisomes have been shown to be essential for full virulence several phytopathogenic fungi. However, the effects changes number pathway on pathogenicity Botry...

In the central nervous system (CNS) peroxisomes are present in all cell types, namely neurons, oligodendrocytes, astrocytes, microglia, and endothelial cells. Brain peroxisomes are smaller in size compared to peroxisomes from other tissues and are therefore referred to as microperoxisomes. We have established a purification procedure to isolate highly purified peroxisomes from the central nervo...

Peroxisomes import virtually all of their membrane and matrix proteins post-translationally. It is presently unknown whether, in mammalian cells, their exists a pool of mature peroxisomes which have received their complement of proteins and are import-incompetent. Previous work has shown that fibroblasts are capable of importing microinjected peroxisomal proteins into peroxisomes. This report d...

The rice blast fungus, Magnaporthe oryzae, is a model plant pathogenic fungus and is a severe threat to global rice production. Over the past two decades, it has been found that the peroxisomes play indispensable roles during M. oryzae infection. Given the importance of the peroxisomes for virulence, we review recent advances of the peroxisomes roles during M. oryzae infection processes. We fir...

Superoxide dismutases (SODs) and superoxide radicals in peroxisomes Peroxisomes are subcellular respiratory organelles that contain catalase and H202-producing flavin oxidases as basic enzymatic constituents [1,2]. In recent years, it has become increasingly clear that peroxisomes carry out essential functions in almost all eukaryotic cells [3-51, and an important characteristic of peroxisomes ...

epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a  chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...

Peroxisomes are essential organelles that are characterized by the possession of enzymes that produce hydrogen peroxide (H2O2) as part of their normal catalytic cycle. During the metabolic process, peroxisomal proteins are inevitably damaged by H2O2 and the integrity of the peroxisomes is impaired. Here, we show that autophagy, an intracellular process for vacuolar degradation, selectively degr...

Peroxisomes can arise de novo from the endoplasmic reticulum (ER) via a maturation process. Peroxisomes can also multiply by fission. We have investigated how these modes of multiplication contribute to peroxisome numbers in Saccharomyces cerevisiae and the role of the dynamin-related proteins (Drps) in these processes. We have developed pulse-chase and mating assays to follow the fate of exist...

Peroxisomes containing fibrillar structures were induced after 1 week withdrawal of bezafibrate, a peroxisome proliferator. In this report, the relation between the duration of bezafibrate treatment and the induction of abnormal peroxisomes in rat hepatocytes was studied morphometrically. The abnormal peroxisomes did not appear during 3 to 90 days of treatment with bezafibrate, but they appeare...