ROS detoxification is an essential process in protecting plant cells and their organelles, which is caused by ROS generated in stress condition. Peroxidase (POD) isozyme, Malondialdehyde (MDA) content, and Ascorbate peroxidase (APX) activity are the indicators of plants for measuring pollution effects. The purpose of the present study was to investigate Cd-induced stress on POD isozymes, MDA co...

Cytochrome P-450 isozyme 3a, isolated from hepatic microsomes of rabbits treated chronically with ethanol, was found to have a unique substrate specificity when compared with isozymes 2, 3b, 3c, and 4. Form 3a has unusually high activity in the p-hydroxylation of aniline and in the oxidation of alcohols to aldehydes. These properties are reflected in the increased activities of these substrates...

104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, a...

The stationary-phase sigma factor, RpoS, influences the expression of factors important in survival of Pseudomonas chlororaphis O6 in the rhizosphere. A partial proteomic profile of a rpoS mutant in P. chlororaphis O6 was conducted to identify proteins under RpoS regulation. Five of 14 differentially regulated proteins had unknown roles. Changes in levels of proteins in P. chlororaphis O6 rpoS ...

Two cu-galactosidase (a-o-galactoside galactohydrolase, EC 3.2.1.22) isozymes have been highly purified from human placental tissue. Both isozymes appeared to be homogeneous based upon disc gel electrophoresis. Both were bound by concanavalin A-Sepharose and stained positively with the periodic acid-Schiff stain indicating that each contained carbohydrate. The A isozyme had a molecular weight o...

Lactate dehydrogenase isozyme 5 from rabbit skeletal muscle is activated by citrate, cis-aconitate, isocitrate, alpha-ketoglutarate, succinate, fumarate, malate, aspartate, and glutamate. In the presence of these activators the shape of the pyruvate saturation curve is changed from sigmoid to hyperbolic. Lactate dehydrogenase isozyme 1 from rabbit heart gives a hyperbolic pyruvate saturation cu...

even purified enzyme preparations are often heterogeneous. for example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme.  for this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the characteristics of the species present.  based...

Methylmercury (MeHg) is an environmental neurotoxicant whose molecular mechanisms underlying toxicity remain elusive. Here, we investigated molecular events involved in MeHg-induced neurotoxicity in cultured cerebellar granule cells (CGCs) as well as potential protective strategies for such toxicity. Glutathione peroxidase, isozyme 1 (GPx-1) activity was significantly (p = 0.0017) decreased at ...

Superoxide dismutase (EC-SOD) controls the level of superoxide in the extracellular space by catalyzing the dismutation of superoxide into hydrogen peroxide and molecular oxygen. In addition, the enzyme reacts with hydrogen peroxide in a peroxidase reaction which is known to disrupt enzymatic activity. Here, we show that the peroxidase reaction supports a site-specific bond cleavage. Analyses b...

The properties of the microbody malate dehydrogenase (EC 1.1.1.37) (MDH) isozyme from cotyledons of Cucumus sativus L. were compared during development. It is concluded that the isozyme remains unaltered, despite the transition from glyoxysomal to peroxisomal function that occurs during greening of the cotyledons. This conclusion is based on electrophoretic behavior, chromatographic elution fro...