Sir, We read with great interest the article on “Comparison of potassium hydroxide mount and mycological culture with histopathological examination using periodic acid-Schiff staining of nail clippings” in a recent issue of IJDVL.[1] The authors’ findings about high sensitivity of periodic acid-Schiff (PAS) staining in the diagnosis of onychomycosis is consistent with previous studies on the su...

AIMS To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue. METHODS Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haematoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxid...

The distribution of neutral mucins, sialomucins and sulphomucins was determined histochemically in the duodenum, jejunum, ileum and colon of the African elephant (Loxodonta africana). The techniques used were periodic acid-Schiff (PAS), alcian blue/periodic acid-Schiff (AB-PAS), high-iron-diamine/alcian blue (HID-AB), alcian blue at varying pH solutions and alcian blue at high temperature after...

Rosetting and non-rosetting lymphocytes collected from normal individuals were stained for the presence of beta-glucuronidase, periodic-acid Schiff activity, gamma glutamyl transpeptidase, acid phosphatase, and alpha-naphthyl butyrate esterase. Lymphocytes which formed rosettes with sheep erythrocytes and non-rosette forming lymphocytes contained cytochemical reaction products for all five stai...

The routine histopathologic stains used for the demonstration of fungi in animal tissue are inadequate. The fungi are differentiated poorly and sometimes not at all. This is a limitation in the diagnosis of the mycotic granulomas in which the organisms may be rare. A definitive fungus stain, therefore, is needed, particularly by the pathologist and mycologist. During an attempt to stain the cap...

1. Oxidation of tissues with performic acid gives rise to histochemically detectable reaction products particularly in two classes of material. These are keratin and lipoids of the phosphatide class. 2. Three methods have been evolved for visualizing the effect of performic acid on cystine-containing structures; two of these (performic acid/Schiff and perfoi-mic acid/cobalt nitrate) also record...

Puroose: Proliferative diabetic retinopathy is assumed to develop because vasogenic factors are released from retinal areas that are ischaemic and hypoxic secondary to occlusion of the retinal vascular bed. It has been propclsed that vascular occlusion in diabetic retinopethy may be due to the deposition of periodic acid Schiff positive glycoprotein compounds in the retinal vascular walls. but ...