conclusions as about 70% of methicillin-resistant isolates belonged to sccmec iii, the present study can conclude that, over an 8-year period, only one dominant and stable clone of mrsa strain was found in tabriz hospitals. this finding could be a result of incorrect medical orientations, inadequate infection controlling policies, and insufficient preventive approaches. objectives the current s...

background: fusobacterium necrophorum as a non-spore-forming gram-negative anaerobic bacillus is an important human and animal pathogen. it may cause severe systemic infections (lemierre's syndrome) and some other infections. the aim of this study was to subtype fusobacterium necrophorum by using pcr methods. materials and methods: twenty five strains of fusobacterium necrophorum subspecies fun...

capsules from a range of pathogenic bacteria are the key determinants of virulency. the capsule hasbeen implicated in virulence of pasteurella multocida. in this study a type-specific polymerase chain reaction(pcr) assay was used for capsular typing of 39 avian p. multocida isolates from iran. the pcr amplified afragment of 1044 bp from all of tested isolates. it was found that all avian p. mul...

in this research a molecular method based on polymerase chain reaction for typing of clostridium perfringens was developed and toxin genotypes of 64 isolates from sheep and goats in iran were determined. the pcr assays were developed for detection of alpha (cpa), beta (cpb) and epsilon (etx) toxin genes, allowing classification of the isolates into genotypes a b, c and d. the field isolates ...

background and objective: shigella species belong to the family enterobacteriaceae, which cause dysentery with abdominal pain and tenesmus in human. our country is one of the endemic and occasional epidemic areas of the disease. in the present study, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) technique that has high differentiation and specificity power compared to ph...

background: rheumatoid arthritis (ra) is the most common chronic inflammatory erosive joint disease with the worldwide distribution of approximately 0.5-1%. molecular methods can be very sensitive in the early stage diagnosis of the disease and therefore prevention the late complications and disabilities. etiology of ra, an autoimmune disease, is not exactly known but immunologic and genetic fa...

background: hla compatibility between transplant donor and recipient is one of the major determinants of transplant outcome. objective: to determine hla class i by pcr- sequence-specific oligonucleotide probe (pcr-ssop) in cord blood donors. methods: genomic dna of 142 cord blood samples registered at the cord blood bank of iran at hematology, oncology, and bone marrow transplantation research ...