In this study, we established a rapid and sensitive method for the detection of viable Salmonella Typhimurium, Staphylococcus aureus, Listeria monocytogenes in milk using biotin-exposure-based immunomagnetic separation (IMS) combined with sodium dodecyl sulfate (SDS), propidium monoazide (PMA), multiplex real-time PCR (mRT-PCR). We used IMS to lessen assay time isolation target bacteria. then o...

zucchini yellow mosaic virus (zymv) is a potyvirus with a worldwide distribution. this virus causes serious economic losses in iran in many cucurbits. during 2002-2003, sam-ples were collected from squash fields in tehran province. five isolates (z1, z2, z3, z4 and z5) were inoculated on 27 species of cucurbitaceae, chenopodiaceae, amarantha-ceae,solanacae, leguminosae and ranunculaceae. chenop...

A simple procedure for isolating yeast DNA suitable for use as a template for PCR amplification is described. SDS treatment alone is sufficient for extraction of chromosomal DNA from yeast cells. Cells of a yeast colony are suspended in a small volume (about 20 microL) of a 0.25% SDS solution, mixed vigorously and centrifuged. The supernatant can be directly used as a template after dilution to...

Spring durum wheat is an important raw material for producing diverse products such as couscous, bulgur, bread, and pasta. The quality of the dough significantly influenced by high-molecular-weight glutenins, allelic status which depends on region breeding program. In this study, a collection 69 cultivars promising lines were analyzed state Glu-A1 Glu-B1 using SDS-PAGE KASP PCR markers. Protein...

Abstract A major challenge in the treatment of infections has been rise extensively drug resistance (XDR) and multidrug (MDR) Acinetobacter baumannii . The goals this study were to determine pattern antimicrobial susceptibility, bla OXA carO genes among burn-isolated A. strains. In study, 100 strains isolated from burn patients their susceptibilities different antibiotics determined using disc ...

Primers were designed from 16S rRNA sequences of Prevotella intermedia sensu stricto and Prevotella nigrescens and were used to discriminate these two species by PCR. The results were compared with those from the PCR technique using primers designed from arbitrarily primed PCR products by Guillot and Mouton (E. Guillot and C. Mouton, J. Clin. Microbiol. 35:1876-1882, 1997). The specificities of...