The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...

Amplified fragment length polymorphism (AFLP) and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genomic fingerprinting assays were compared for their ability to differentiate Escherichia coli isolates obtained from various host sources, and with respect to their pathogenicity. One hundred and ten verotoxigenic, enterotoxigenic and non-pathogenic E. coli is...

conclusions l. innocua is not a pathogen, but the presence of the bacterium could be an indicator of probable contamination with l. monocytogenes. moreover, there is a potential risk to public health from the consumption of raw or undercooked burgers, which may increase the possibility of the acquisition of resistance to antibiotics. objectives the aim of this study was to evaluate the occurren...

purpose: to ascertain whether the use of nested-pcr (nested polymerase chain reaction) technique leads to more rapid and accurate diagnosis of bacterial endophthalmitis. methods: the aqueous humor and vitreous samples of 24 eyes of 24 patients with bacterial endophthalmitis were evaluated by microscopy, diagnostic aerobic culture, and nested- pcr to detect the infectious agent. results: aerobic...

today, it has been confirmed that the viral infections play an important role in premature delivery and abortion. this study was carried out to determine the frequency rate of papilloma virus types 16 and 18 in pregnant women and their relationship with premature delivery and abortion. in this study, vaginal secretions were collected from pregnant women who had been referred to the women clinic...

A minute of silence. Kary Banks Mullis has died (08/07/2019). was an American Biochemist and Doctor Chemistry who developed the Polymerase Chain Reaction (PCR) in 1985, considered one most important legacies history Humanity, along with description DNA (Watson, Crick Franklin) theory Relativity (Einstein). Currently, I consider anyone does not know PCR by to be illiterate, since it is a molecul...